{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:55:24Z","timestamp":1790661324269,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004508","name":"Prince of Songkla University","doi-asserted-by":"publisher","award":["MET6202037S"],"award-info":[{"award-number":["MET6202037S"]}],"id":[{"id":"10.13039\/501100004508","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004508","name":"Prince of Songkla University","doi-asserted-by":"publisher","award":["MET6402028S"],"award-info":[{"award-number":["MET6402028S"]}],"id":[{"id":"10.13039\/501100004508","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Macrophages play essential roles in erythrophagocytosis and iron recycling. \u03b2\u2010thalassemia is characterized by a genetic defect in hemoglobin synthesis, which increases the rate of iron recycling. We previously showed that reduced expression of the BTB and CNC homolog 1 (BACH1) gene leads to increased phagocytosis of abnormal RBCs by activated monocytes. However, the mechanisms underlying this abnormal RBC clearance remained unclear. Herein, the spleen and bone marrow cells of \u03b2\u2010thalassemic mice were examined for erythrophagocytosis CD markers and iron\u2010recycling genes. Higher expression levels of CD47 and CD163 on RBCs and macrophages, respectively, were observed in \u03b2\u2010thalassemic mice than in wild\u2010type cells. The decreased expression of <jats:italic>BACH1<\/jats:italic> caused an increase in <jats:italic>Nrf2, Spic<\/jats:italic>, <jats:italic>Slc40a1<\/jats:italic>, and <jats:italic>HMOX1<\/jats:italic> expression in splenic red pulp macrophages of thalassemic mice. To investigate BACH1 regulation, a macrophage cell line was transfected with BACH1\u2010siRNA. Decreased <jats:italic>BACH1<\/jats:italic> expression caused an increase in CD163 expression; however, the expression levels were lower when the cells were cultured in media supplemented with \u03b2\u2010thalassemia\/HbE patient plasma. Additionally, the iron recycling\u2010related genes <jats:italic>SPIC<\/jats:italic>, <jats:italic>SLC40A1<\/jats:italic>, and <jats:italic>HMOX1<\/jats:italic> were significantly upregulated in BACH1\u2010suppressed macrophages. Our findings provide insights into BACH1 regulation, which plays an important role in erythrophagocytosis and iron recycling in thalassemic macrophages.<\/jats:p>","DOI":"10.1111\/gtc.13004","type":"journal-article","created":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T15:54:16Z","timestamp":1671897256000},"page":"211-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>BACH1<\/scp> regulates erythrophagocytosis and iron\u2010recycling in \u03b2\u2010thalassemia"],"prefix":"10.1111","volume":"28","author":[{"given":"Tipparat","family":"Penglong","sequence":"first","affiliation":[{"name":"Department of Pathology, Faculty of Medicine Prince of Songkla University  Songkhla Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apisara","family":"Saensuwanna","sequence":"additional","affiliation":[{"name":"Faculty of Medical Technology Prince of Songkla University  Songkhla Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuttanan","family":"Pholngam","sequence":"additional","affiliation":[{"name":"Molecular Medicine Graduate Program, Faculty of Science Mahidol University  Bangkok Thailand"},{"name":"Thalassemia Research Center, Institute of Molecular Biosciences Mahidol University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natta","family":"Tansila","sequence":"additional","affiliation":[{"name":"Faculty of Medical Technology Prince of Songkla University  Songkhla Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hansuk","family":"Buncherd","sequence":"additional","affiliation":[{"name":"Faculty of Medical Technology Prince of Songkla University  Songkhla 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