{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T20:19:31Z","timestamp":1778703571703,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21gm1010004"],"award-info":[{"award-number":["JP21gm1010004"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["JP18K15187"],"award-info":[{"award-number":["JP18K15187"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["JP21H050430"],"award-info":[{"award-number":["JP21H050430"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the intestine, interleukin (IL)\u201023 and IL\u201022 from immune cells in the lamina propria contribute to maintenance of the gut epithelial barrier through the induction of antimicrobial production and the promotion of epithelial cell proliferation. Several previous studies suggested that some of the functions of the IL\u201023\/IL\u201022 axis on intestinal epithelial cells are shared between the small and large intestines. However, the similarities and differences of the IL\u201023\/IL\u201022 axis on epithelial cells between these two anatomical sites remain unclear. Here, we comprehensively analyzed the gene expression of intestinal epithelial cells in the ileum and colon of germ\u2010free, <jats:italic>Il23<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>, and <jats:italic>Il22<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice by RNA\u2010sequencing. We found that while the IL\u201023\/IL\u201022 axis is largely dependent on gut microbiota in the small intestine, it is much less dependent on it in the large intestine. In addition, the negative regulation of lipid metabolism in the epithelial cells by IL\u201023 and IL\u201022 in the small intestine was revealed, whereas the positive regulation of epithelial cell proliferation by IL\u201023 and IL\u201022 in the large intestine was highlighted. These findings shed light on the intestinal site\u2010specific role of the IL\u201023\/IL\u201022 axis in maintaining the physiological functions of intestinal epithelial cells.<\/jats:p>","DOI":"10.1111\/gtc.13065","type":"journal-article","created":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T06:35:47Z","timestamp":1694154947000},"page":"776-788","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differential dependence on microbiota of <scp>IL<\/scp>\u201023\/<scp>IL<\/scp>\u201022\u2010dependent gene expression between the small\u2010 and large\u2010intestinal epithelia"],"prefix":"10.1111","volume":"28","author":[{"given":"Ayaka","family":"Nakatani","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine Osaka University  Suita, Osaka Japan"},{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, Graduate School of Medicine Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryu","family":"Okumura","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine Osaka University  Suita, Osaka Japan"},{"name":"WPI Immunology Frontier Research Center Osaka University  Suita, Osaka Japan"},{"name":"Institute for Open and Transdisciplinary Research Initiative Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Airi","family":"Ishibashi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shota","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kei","family":"Sakaki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Okuzaki","sequence":"additional","affiliation":[{"name":"WPI Immunology Frontier Research Center Osaka University  Suita, Osaka Japan"},{"name":"Institute for Open and Transdisciplinary Research Initiative Osaka University  Suita, Osaka Japan"},{"name":"Genome Information Research Center, Research Institute for Microbial Diseases Osaka University  Suita, Osaka Japan"},{"name":"Center for Infectious Disease Education and Research Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Inohara","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, Graduate School of Medicine Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1778-6332","authenticated-orcid":false,"given":"Kiyoshi","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Graduate School of Medicine Osaka University  Suita, Osaka Japan"},{"name":"WPI Immunology Frontier Research Center Osaka University  Suita, Osaka Japan"},{"name":"Institute for Open and Transdisciplinary Research Initiative Osaka University  Suita, Osaka Japan"},{"name":"Center for Infectious Disease Education and Research Osaka University  Suita, Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20150318"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.08.058"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.09.011"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2011.09.008"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2486-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1307-8_22"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-020-01474-7"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0899-7"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.05.028"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.09.033"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-070119-115104"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20192195"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.12.011"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20122308"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20161076"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.03.010"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16460"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-020-01304-w"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10010111"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25437"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-19-1086"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.399998"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17406"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2017.20"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16363-w"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.08.007"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.12.047"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI33194"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2021.03.010"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2100594118"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.05.009"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209791"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.9"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13564"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-20-4192"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1856-y"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1720"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20480"}],"container-title":["Genes 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