{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T20:30:20Z","timestamp":1764880220970,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18H05528","JP21J00155"],"award-info":[{"award-number":["JP18H05528","JP21J00155"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple method for producing pseudopregnant mice supports pup production. In this study, pregnant ICR were obtained mice without mating with vasectomized mice via administration of mouse Kisspeptin\u201010 (mKp\u201010) and transferring blastocysts to the uterus. Blastocyst transfer after mKp\u201010 administration to mice with gapping and reddish pink vagina resulted in 65.2% (15\/23) pregnancies, and 39.1% (34\/87) of the transferred blastocysts showed full\u2010term growth. Vaginal smears were observed for accurate estrus cycle determination, and subsequent administration of mKp10 to mice during the estrus stage and blastocyst transfer resulted in 95.2% (20\/21) pregnancies and 50.7% (104\/205) birth rates. Regarding 2\u2010cell transfer after administration of mKp\u201010, 100% (8\/8) of the mice became pregnant, and 45.0% (36\/80) of the embryos were born. Administration of mKp\u201010 to mice during the estrus stage is a convenient way to generate pseudopregnant mice.<\/jats:p>","DOI":"10.1111\/gtc.13077","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T08:30:09Z","timestamp":1698395409000},"page":"906-914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Intraperitoneal administration of mouse kisspeptin\u201010 to mice during estrus stage induces pseudopregnancy"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-3076","authenticated-orcid":false,"given":"Daisuke","family":"Mashiko","sequence":"first","affiliation":[{"name":"Graduate School of Biology\u2010Oriented Science and Technology Kindai University  Wakayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikiko","family":"Tokoro","sequence":"additional","affiliation":[{"name":"Graduate School of Biology\u2010Oriented Science and Technology Kindai University  Wakayama Japan"},{"name":"Asada Institute for Reproductive Medicine, Asada Ladies Clinic  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuma","family":"Yao","sequence":"additional","affiliation":[{"name":"Graduate School of Biology\u2010Oriented Science and Technology Kindai University  Wakayama Japan"},{"name":"Research and Development Center, Fuso Pharmaceutical Industries, Ltd.  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