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One of the factors regulating this heterogeneity is the chromatin state in each cell. However, the mechanisms of epigenetic chromatin regulation of specific chromosomal regions remain unclear. Therefore, we used single\u2010cell tracking system to analyze <jats:italic>IMD2<\/jats:italic>. <jats:italic>IMD2<\/jats:italic> is located at the subtelomeric region of budding yeast, and its expression is epigenetically regulated by heterochromatin fluctuations. Treatment with mycophenolic acid, an inhibitor of de novo GTP biosynthesis, triggered a decrease in GTP, which caused heterochromatin fluctuations at the <jats:italic>IMD2<\/jats:italic> locus. Interestingly, within individually tracked cells, <jats:italic>IMD2<\/jats:italic> expression state underwent repeated switches even though <jats:italic>IMD2<\/jats:italic> is positioned within the heterochromatin region. We also found that 30% of the cells in a population always expressed <jats:italic>IMD2<\/jats:italic>. Furthermore, the addition of nicotinamide, a histone deacetylase inhibitor, or guanine, the GTP biosynthesis factor in salvage pathway of GTP biosynthesis, regulated heterogeneity, resulting in <jats:italic>IMD2<\/jats:italic> expression being uniformly induced or suppressed in the population. These results suggest that gene expression heterogeneity in the <jats:italic>IMD2<\/jats:italic> region is regulated by changes in chromatin structure triggered by slight decreases in GTP.<\/jats:p>","DOI":"10.1111\/gtc.13094","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T05:15:11Z","timestamp":1705468511000},"page":"217-230","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>GTP<\/scp>\u2010dependent regulation of heterochromatin fluctuations at subtelomeric regions in <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1111","volume":"29","author":[{"given":"Takahito","family":"Ayano","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry and Biotechnology, Graduate School of Engineering University of Fukui  Fukui Japan"},{"name":"Research Fellowships of Japan Society for the Promotion of Science for Young Scientists (JSPS)  Tokyo 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