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We established a lung adenocarcinoma cell subline (H322L\u2010BO4) that specifically showed metastasis to the leg bones and adrenal glands. This was achieved by repeated isolation of metastatic cells from the leg bones of mice. The cells were intracardially injected into nude mice. Survival was prolonged for mice that received H322L\u2010BO4 cells versus original cells (H322L). H322L\u2010BO4 cells did not exhibit obvious changes in general in vitro properties associated with the metastatic potential (e.g., cell growth, migration, and invasion) compared with H322L cells. However, the phosphorylation of chromosome 9 open reading frame 10\/oxidative stress\u2010associated Src activator (C9orf10\/Ossa) was increased in H322L\u2010BO4 cells. This result confirmed the increased anchorage independence through C9orf10\/Ossa\u2010mediated activation of Src family tyrosine kinase. Reduction of C9orf10\/Ossa by shRNA reduced cells' metastasis to the leg bone and prolonged survival in mice. These findings indicate that H322L\u2010BO4 cells can be used to evaluate the effect of candidate therapeutic targets against bone metastatic lung cancer cells. Moreover, C9orf10\/Ossa may be a useful target for treatment of lung cancer with bone metastasis.<\/jats:p>","DOI":"10.1111\/gtc.13103","type":"journal-article","created":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T10:45:21Z","timestamp":1707561921000},"page":"290-300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["C9orf10\/Ossa regulates the bone metastasis of established lung adenocarcinoma cell subline <scp>H322L\u2010BO4<\/scp> in a mouse model"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8491-7849","authenticated-orcid":false,"given":"Takamasa","family":"Uekita","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry National Defense Academy  Yokosuka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiko","family":"Yagi","sequence":"additional","affiliation":[{"name":"Division of Metastasis and Invasion Signaling 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