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In HeLa cells, the HPV18 viral genome is integrated at chromosome 8q24.21 and activates transcription of the proto\u2010oncogene <jats:italic>c\u2010Myc<\/jats:italic>. However, the mechanism of how the integrated HPV genome and its transcribed RNAs exhibit transcription activation function has not been fully elucidated. In this study, we found that HPV18 transcripts contain an enhancer RNA\u2010like function to activate proximal genes including <jats:italic>CCAT1\u20105L<\/jats:italic> and <jats:italic>c\u2010Myc<\/jats:italic>. We showed that the human genome\u2010integrated <jats:italic>HPV18<\/jats:italic> genes are activated by transcription coregulators including BRD4 and Mediator. The transcribed HPV18 RNAs form a liquid\u2010like condensate at chromosome 8q24.21 locus, which in turn accumulates RNA polymerase II. Moreover, we focused on a relatively uncharacterized transcript from the <jats:bold>u<\/jats:bold>pstream <jats:bold>r<\/jats:bold>egion of <jats:italic><jats:bold>C<\/jats:bold>CAT1<\/jats:italic>, named <jats:italic>URC<\/jats:italic>. The <jats:italic>URC<\/jats:italic> RNA is transcribed as a chimera RNA with HPV18 and is composed of the 3\u2032\u2010untranslated region of the HPV18 transcript. We experimentally showed that the <jats:italic>URC<\/jats:italic> contributes to stabilization of HPV18 RNAs by supplying a polyadenylation site for the HPV18 transcript. Our findings suggest that integrated HPV18 at 8q24.21 locus produces HPV18\u2010URC chimera RNA and promotes tumorigenesis through RNA\u2010based condensate formation.<\/jats:p>","DOI":"10.1111\/gtc.13121","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T04:04:23Z","timestamp":1715141063000},"page":"532-548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chimera <scp>RNA<\/scp> transcribed from integrated <scp>HPV18<\/scp> genome with adjacent host genomic region promotes oncogenic gene expression through condensate formation"],"prefix":"10.1111","volume":"29","author":[{"given":"Kazuki","family":"Furugori","sequence":"first","affiliation":[{"name":"Department of Molecular Biology Yokohama City University Graduate School of Medical Science  Yokohama Kanagawa 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