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Diabetic cataracts occur at young age and progress relatively quickly, so the development of effective treatment has been awaited. Several studies have shown that pyruvate inhibits oxidative stress and glycation of lens proteins, which contribute to onset of diabetic cataracts. However, detailed molecular mechanisms have not been revealed. In this study, we attempted to reduce galactose\u2010induced opacity by pyruvate with rat ex vivo model. Rat lenses were extracted and cultured in galactose\u2010containing medium to induce lens opacity. After opacity had developed, continued culturing with pyruvate in the medium resulted in a reduction of lens opacity. Subsequently, we conducted microarray analysis to investigate the genes that contribute to the therapeutic effect. We performed quantitative expression measurements using RT\u2010qPCR for extracted genes that were upregulated in cataract\u2010induced lenses and downregulated in pyruvate\u2010treated lenses, resulting in the identification of 34 candidate genes. Functional analysis using the STRING database suggests that metallothionein\u2010related factors (\n                    <jats:italic>Mt1a<\/jats:italic>\n                    ,\n                    <jats:italic>Mt1m<\/jats:italic>\n                    , and\n                    <jats:italic>Mt2A<\/jats:italic>\n                    ) and epithelial\u2010mesenchymal transition\u2010related factors (\n                    <jats:italic>Acta2<\/jats:italic>\n                    ,\n                    <jats:italic>Anxa1<\/jats:italic>\n                    ,\n                    <jats:italic>Cd81<\/jats:italic>\n                    ,\n                    <jats:italic>Mki67<\/jats:italic>\n                    ,\n                    <jats:italic>Timp1<\/jats:italic>\n                    , and\n                    <jats:italic>Tyms<\/jats:italic>\n                    ) contribute to the therapeutic effect of cataracts.\n                  <\/jats:p>","DOI":"10.1111\/gtc.13150","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T02:20:00Z","timestamp":1725243600000},"page":"876-888","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["EXPRESSION OF CONCERN: Identification of genes contributing to attenuation of rat model of galactose\u2010induced cataract by pyruvate"],"prefix":"10.1111","volume":"29","author":[{"given":"Fuuga","family":"Masuda","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry and Biotechnology, Graduate School of Engineering University of Fukui  Fukui Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayumi","family":"Inami","sequence":"additional","affiliation":[{"name":"Technical Division, School of Engineering University of Fukui  Fukui 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