{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:11:31Z","timestamp":1747185091268,"version":"3.40.5"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18H04034","22H00458","22H04996"],"award-info":[{"award-number":["18H04034","22H00458","22H04996"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Completion of DNA replication before chromosome segregation is essential for the stable maintenance of the genome. Under replication stress, DNA synthesis may persist beyond S phase, especially in genomic regions that are difficult to proceed with the replication processes. Incomplete replication in mitosis emerges as non\u2010disjoined segment in mitotic chromosomes leading to anaphase bridges. The resulting chromosome rearrangements are not well characterized, however. Here, we report that incomplete replication due to SMC5\/6 deficiency impairs sister chromatid disjunction at difficult\u2010to\u2010replicate regions, including common fragile sites. These non\u2010disjoined regions manifest as cytologically defined symmetric gaps, causing anaphase bridges. These bridges break at the gaps, leading to telomere loss, micronucleation, and fragmentation. Subsequently, fusions between telomere\u2010deficient chromosomes generate complex chromosomal rearrangements, including dicentric chromosomes, suggesting the occurrence of breakage\u2010fusion\u2010bridge cycle. Additionally, chromosomes in micronuclei were pulverized, indicative of chromothripsis. Our findings suggest that incomplete replication facilitates complex chromosomal rearrangements, which may contribute to genomic instability in human cancers.<\/jats:p>","DOI":"10.1111\/gtc.13180","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T10:00:42Z","timestamp":1731578442000},"page":"1251-1263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chromosomal rearrangements associated with <scp>SMC5<\/scp>\/6 deficiency in <scp>DNA<\/scp> replication"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9806-5077","authenticated-orcid":false,"given":"Yoshiharu","family":"Kusano","sequence":"first","affiliation":[{"name":"Division of Experimental Pathology Cancer Institute of the Japanese Foundation for Cancer Research (JFCR)  Tokyo Japan"},{"name":"Department of JFCR Cancer Biology, Graduate School of Medical and Dental Sciences Institute of Science Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuha","family":"Kinugasa","sequence":"additional","affiliation":[{"name":"Research Institute for Radiation Biology and Medicine Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Tashiro","sequence":"additional","affiliation":[{"name":"Research Institute for Radiation Biology and Medicine Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Hirota","sequence":"additional","affiliation":[{"name":"Division of Experimental Pathology Cancer Institute of the Japanese Foundation for Cancer Research (JFCR)  Tokyo Japan"},{"name":"Department of JFCR Cancer Biology, Graduate School of Medical and Dental Sciences Institute of Science Tokyo  Tokyo 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