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Although multiple transcript variants of\n                    <jats:italic>Sox17<\/jats:italic>\n                    have been suggested, the precise mechanisms underlying their time\u2010 and tissue\u2010specific expression remain unclear. In this study, we discovered two putative regulatory sequences (R1 and R2) adjacent to different transcription start sites of mouse\n                    <jats:italic>Sox17<\/jats:italic>\n                    exon 1 and generated deletion mice for these regions (\n                    <jats:italic>Sox17<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u0394dr\/\u0394dr<\/jats:italic>\n                    <\/jats:sup>\n                    <jats:italic>)<\/jats:italic>\n                    .\n                    <jats:italic>Sox17<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u0394dr\/\u0394dr<\/jats:italic>\n                    <\/jats:sup>\n                    mice were alive and fertile, and they possessed a normal\u2010sized gallbladder. However, semiquantitative analysis of immunostaining showed that the expression levels of SOX17 in\n                    <jats:italic>Sox17<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u0394dr\/\u0394dr<\/jats:italic>\n                    <\/jats:sup>\n                    embryos were reduced to less than 50% of the wild\u2010type in the gallbladder epithelium. Furthermore, the bile ductal epithelium marker SOX9 was abnormally upregulated, and PAS\/DBA\u2010positive mucin secretion\u2010like epithelial cells were induced in the\n                    <jats:italic>Sox17<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u0394dr\/\u0394dr<\/jats:italic>\n                    <\/jats:sup>\n                    gallbladder. Our results demonstrate that the distal sequence of\n                    <jats:italic>Sox17<\/jats:italic>\n                    , including R1 and R2, is important for the regulation of\n                    <jats:italic>Sox17<\/jats:italic>\n                    gene expression in the embryonic gallbladder and is crucial for normal gallbladder epithelial development.\n                  <\/jats:p>","DOI":"10.1111\/gtc.13186","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:34Z","timestamp":1735257634000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Gallbladder\u2010Specific Distal Regulatory Sequence of Murine Sox17"],"prefix":"10.1111","volume":"30","author":[{"given":"Shihan","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Veterinary Anatomy The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6350-2185","authenticated-orcid":false,"given":"Ayaka","family":"Yanagida","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriaki","family":"Ota","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mami","family":"Uemura","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy The University of Tokyo  Tokyo Japan"},{"name":"Research Center for Biological Products in the Next Generation National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Hirate","sequence":"additional","affiliation":[{"name":"Department of Experimental Animal Model for Human Disease Institute of Science Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuji","family":"Hiramatsu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoaki","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Department of Experimental Animal Model for Human Disease Institute of Science Tokyo  Tokyo Japan"},{"name":"Stem Cell Therapy Division Institute of Science Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiakira","family":"Kanai","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masami","family":"Kanai\u2010Azuma","sequence":"additional","affiliation":[{"name":"Department of Experimental Animal Model for Human Disease Institute of Science Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9883"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1192"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.20-00032"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.088765"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20540"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.060146"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh458"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.147512"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24171"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.3.667"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.10.2367"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.06.011"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx116"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20520"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0560-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03081"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.11.1046"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24466"},{"key":"e_1_2_9_20_1","volume-title":"RStudio: Integrated Development for R. 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