{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T15:19:18Z","timestamp":1782573558510,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K21385"],"award-info":[{"award-number":["JP20K21385"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K18221"],"award-info":[{"award-number":["JP23K18221"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Calcium\/calmodulin\u2010dependent protein kinase II\u03b4 (CaMKII\u03b4), encoded by the\n                    <jats:italic>Camk2d<\/jats:italic>\n                    gene, plays key regulatory roles in various Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010regulated cellular processes. Extensive alternative splicing of the\n                    <jats:italic>Camk2d<\/jats:italic>\n                    gene generates multiple CaMKII\u03b4 splice variants that exhibit differential roles. Despite significant advances in understanding the functions of CaMKII\u03b4, the full repertoire of\n                    <jats:italic>Camk2d<\/jats:italic>\n                    splice variants in a variety of tissues and their distinct roles in physiological and pathological contexts remain incompletely characterized due to the complex nature of multiple alternative splicing events. Here, we conducted long\u2010read amplicon sequencing to investigate the murine\n                    <jats:italic>Camk2d<\/jats:italic>\n                    splice variants in the heart, skeletal muscle, and olfactory bulbs and show that mRNAs in the heart and skeletal muscle have shorter 3'UTRs. Our results in this study suggest that a key regulator of\n                    <jats:italic>Camk2d<\/jats:italic>\n                    splicing, RNA\u2010binding motif protein 20 (RBM20), whose\n                    <jats:italic>gain\u2010of\u2010function<\/jats:italic>\n                    mutations cause dilated cardiomyopathy, is crucial for the expression of heart\u2010specific splice variants. Olfactory bulbs specifically express novel splice variants that utilize a mutually exclusive exon 6B and\/or an alternative polyadenylation site in a novel exon 17.5 in an RBM20\u2010independent manner. The tissue\u2010specific repertoire of CaMKII\u03b4 splice variants and their aberrant expression in disease model animals will help in understanding their roles in physiological and pathological contexts.\n                  <\/jats:p>","DOI":"10.1111\/gtc.70021","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T05:20:16Z","timestamp":1746681616000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Profiling of\n                    <scp>RBM20<\/scp>\n                    \u2010Regulated\n                    <scp>CaMKII\u03b4<\/scp>\n                    Splice Variants Across the Heart, Skeletal Muscle, and Olfactory Bulbs"],"prefix":"10.1111","volume":"30","author":[{"given":"Yui","family":"Maeda","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Graduate School of Medicine University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Yamasu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Graduate School of Medicine University of the Ryukyus  Okinawa Japan"},{"name":"Life Science and Technology Track, Graduate School of Medical and Dental Sciences Institute of Science Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1563-1134","authenticated-orcid":false,"given":"Hidehito","family":"Kuroyanagi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Graduate School of Medicine University of the Ryukyus  Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010015\u20100653\u2010x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2011.01.012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.05.033"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvw192"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.05.038"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.644630"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2021.11.022"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201026623\u2010y"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.24096"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2014.00015"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2017.01.002"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2693"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.110601.135410"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201074800\u20108"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201039965\u20106"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11897\u2010020\u201000475\u2010x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.04.128"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.163006"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010022\u201004624\u20104"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.316947"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.113.005610"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201026624\u2010w"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.ade1633"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1189"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1653"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae974"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010022\u201001565\u2010y"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010020\u20101087\u2010x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaz0240"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.126.4.839"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.117.031947"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2022.01.004"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2018.00105"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.02.013"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv468"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.11.036"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109\u2010022\u201002262\u20108"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010019\u20100380\u20108"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108525200"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202101811RR"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201024484\u2010z"}],"container-title":["Genes 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