{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:46:42Z","timestamp":1787237202363,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007802","name":"Institute for Fermentation, Osaka","doi-asserted-by":"publisher","award":["G\u20102019\u20102\u2010145"],"award-info":[{"award-number":["G\u20102019\u20102\u2010145"]}],"id":[{"id":"10.13039\/100007802","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K15752"],"award-info":[{"award-number":["JP20K15752"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The fission yeast\n                    <jats:italic>Schizosaccharomyces pombe<\/jats:italic>\n                    is a valuable unicellular model organism that proliferates predominantly in the haploid state. Under nitrogen starvation, sexual reproduction occurs, resulting in the formation of a diploid zygote and subsequent meiosis, producing four haploid ascospores. The genetic tractability of yeast, particularly, its ability to produce offspring through sexual reproduction, makes it a widely used model organism. Spores also serve as a model for dormant cells. In this study, I present a highly efficient and low\u2010cost method for purifying\n                    <jats:italic>S. pombe<\/jats:italic>\n                    spores using an aqueous two\u2010phase system (ATPS). Using polyethylene glycol (PEG)\u2010salt (e.g., phosphate)\u2010based ATPS, free spores were found to partition exclusively into the upper (PEG\u2010rich) phase. In contrast, spores lacking Isp3, which forms the outermost spore wall layer, partitioned into the lower (salt\u2010rich) phase, like vegetative cells. This suggests that the Isp3 layer imparts hydrophobicity to the spore surface, facilitating efficient separation in ATPS. This unique surface property may also reflect differences in ecological adaptation and spore dispersal strategies between\n                    <jats:italic>S. pombe<\/jats:italic>\n                    and other fission yeast species.\n                  <\/jats:p>","DOI":"10.1111\/gtc.70029","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T04:03:51Z","timestamp":1748837031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Aqueous Two\u2010Phase System (ATPS)\u2010Based Spore Isolation in\n                    <i>Schizosaccharomyces pombe<\/i>\n                    Requires Isp3\u2010Dependent Surface Hydrophobicity"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9894-6579","authenticated-orcid":false,"given":"Kazuki","family":"Imada","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry  Suzuka Mie Japan"},{"name":"Department of Biology Graduate School of Science, Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102958.2000.01678.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.06.051"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1347"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e13\u201012\u20100731"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12575\u2010016\u20100048\u20108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287\u2010115\u20101\u2010255"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.04.008"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.68.1489"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/m78\u2010149"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1303"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203357"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2405553121"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00415605"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2007.08.003"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7010007"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015060\u2010y"},{"key":"e_1_2_9_18_1","first-page":"1037","volume-title":"The Molecular and Cellular Biology of the Yeast Saccharomyces","author":"Yamamoto M.","year":"1997"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00409533"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001597"}],"container-title":["Genes to Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/gtc.70029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T07:55:34Z","timestamp":1753430134000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/gtc.70029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,2]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/gtc.70029"],"URL":"https:\/\/doi.org\/10.1111\/gtc.70029","archive":["Portico"],"relation":{},"ISSN":["1356-9597","1365-2443"],"issn-type":[{"value":"1356-9597","type":"print"},{"value":"1365-2443","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,2]]},"assertion":[{"value":"2025-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70029"}}