{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:15:12Z","timestamp":1773008112824,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K07926"],"award-info":[{"award-number":["15K07926"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Var2CSA, a member of the\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    erythrocyte membrane protein (PfEMP) family, specifically binds to various human cancer cells through oncofetal chondroitin sulfate (ofCS) expressed on cell surfaces. This unique binding property has raised considerable expectations for the application of var2CSA in cancer\u2010targeted diagnostics and therapies. Baculovirus vectors, known for their safety compared with other viral vectors, possess the ability to introduce genes into human cancer cells. We previously constructed a baculovirus expressing var2CSA containing DBL1X\u2010DBL3X domains (var2CSA\u2010type baculovirus) and demonstrated enhanced gene transduction efficiency in cancer cells. In the present study, we sought to further improve gene delivery by generating two truncated var2CSA constructs: rVAR2 (DBL1X\u2010ID2a) and ID1\u2010DBL2Xb. The rVAR2 was successfully expressed in purified budded virions, whereas the ID1\u2010DBL2Xb showed no detectable expression in budded virions due to very low protein levels in insect Sf9 cells. Functional evaluation in HepG2 hepatocellular carcinoma cells revealed that rVAR2\u2010type baculovirus exhibited significantly higher gene delivery efficiency than the var2CSA\u2010type baculovirus. These findings suggest that rVAR2\u2010expressing baculovirus represents a promising tool for cancer gene therapy, where enhanced gene delivery efficiency is highly desirable.\n                  <\/jats:p>","DOI":"10.1111\/gtc.70081","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T12:07:17Z","timestamp":1767787637000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of Truncated Malarial\n                    <scp>Var2CSA<\/scp>\n                    \u2010Displaying Baculovirus Vectors and Their Gene Delivery Efficiency in\n                    <scp>HepG2<\/scp>\n                    Cells"],"prefix":"10.1111","volume":"31","author":[{"given":"Chiaki","family":"Kawabata","sequence":"first","affiliation":[{"name":"School of Pharmacy Kanazawa University  Kanazawa Ishikawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benoit","family":"Gamain","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, UMR_S1134, BIGR, INSERM  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2869-9901","authenticated-orcid":false,"given":"Takahiko","family":"Tamura","sequence":"additional","affiliation":[{"name":"School of Pharmacy Kanazawa University  Kanazawa Ishikawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05793-2"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-092917-043356"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.348839"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.70106"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.634508"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/88122"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2010.25"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b22-00630"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b20-00684"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b18-00451"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2018.05.002"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135406"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15010080"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2015.09.003"},{"key":"e_1_2_12_16_1","first-page":"115","article-title":"Baculoviruses in Gene Therapy and Personalized Medicine","volume":"15","author":"Schaly S.","year":"2021","journal-title":"Biologics"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020270"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000951107"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.2879"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07025-6"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.70067"}],"container-title":["Genes to 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