{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T04:04:35Z","timestamp":1779681875265,"version":"3.53.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010795","name":"Chugai Pharmaceutical","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Neovascularization is a major cause of blindness in various retinal diseases, and inflammation aggravates the pathological and clinical conditions of these diseases. VEGFA or ANG\u20102 neutralizing antibodies have been used to block pathological neovascularization. In this work, the effects of intraocular administration of neutralizing antibodies against VEGFA, ANG\u20102, or bispecific to these two factors on pathological findings were examined in the oxygen\u2010induced retinopathy (OIR) mouse model. At both postnatal day (P)17 and P19, anti\u2010VEGFA and \u2010ANG\u20102 administration suppressed neovascularization, and the bispecific antibody attenuated neovascularization more efficiently. However, oxygen\u2010induced vaso\u2010obliteration was not modified by these antibodies. Numbers of photoreceptor, amacrine, and bipolar cells were reduced in the OIR retina, and the antibodies reversed these changes. Microglia\u2010specific gene expression increased in the OIR retina, and administration of the antibodies reduced the IBA1\u2010positive area in the OIR retina, although these antibodies did not affect\n                    <jats:italic>Iba1<\/jats:italic>\n                    gene expression. Labeled VEGFA and ANG\u20102 were found to be co\u2010localized with microglia, suggesting that VEGFA and ANG\u20102 affect microglia activation directly. Taken together, neutralizing antibody to VEGFA or ANG\u20102 attenuated oxygen\u2010induced neovascularization and inflammation, and the bispecific antibody more efficiently suppressed some features than the single antibody to VEGFA or ANG\u20102.\n                  <\/jats:p>","DOI":"10.1111\/gtc.70107","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T14:49:50Z","timestamp":1775573390000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Attenuation of Oxygen\u2010Induced Neovascularization and Inflammation by Neutralizing\n                    <scp>VEGFA<\/scp>\n                    and\/or\n                    <scp>ANG<\/scp>\n                    \u20102 With an Antibody"],"prefix":"10.1111","volume":"31","author":[{"given":"Hirokazu","family":"Oohashi","sequence":"first","affiliation":[{"name":"Department of Retinal Biology and Pathology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"},{"name":"Graduate School of Pharmaceutical Sciences Josai International University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1600-7795","authenticated-orcid":false,"given":"Toshiro","family":"Iwagawa","sequence":"additional","affiliation":[{"name":"Department of Retinal Biology and Pathology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroto","family":"Abe","sequence":"additional","affiliation":[{"name":"Product Research Dept Chugai Pharmaceutical Co., Ltd.  Togane Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Kawaji","sequence":"additional","affiliation":[{"name":"Department of Retinal Biology and Pathology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2385-3505","authenticated-orcid":false,"given":"Yuta","family":"Inokuchi","sequence":"additional","affiliation":[{"name":"Department of Retinal Biology and Pathology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"},{"name":"Product Research Dept Chugai Pharmaceutical Co., Ltd.  Togane Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuhiro","family":"Soeda","sequence":"additional","affiliation":[{"name":"Product Research Dept Chugai Pharmaceutical Co., Ltd.  Togane Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Saita","sequence":"additional","affiliation":[{"name":"Department of Retinal Biology and Pathology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Aihara","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4497-5750","authenticated-orcid":false,"given":"Sumiko","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Retinal Biology and Pathology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"},{"name":"Department of Ophthalmology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"1647","article-title":"Vascular Endothelial Growth Factor. 20th\u2010Century Mechanisms, 21st\u2010Century Therapies","volume":"38","author":"Aiello L. 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