{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:44:25Z","timestamp":1790639065090,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K18381"],"award-info":[{"award-number":["JP23K18381"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP25H00755"],"award-info":[{"award-number":["JP25H00755"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23fk0108669"],"award-info":[{"award-number":["JP23fk0108669"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23fk0108667"],"award-info":[{"award-number":["JP23fk0108667"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["JPMH25KA1001"],"award-info":[{"award-number":["JPMH25KA1001"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Human norovirus (HuNoV) is a major cause of acute viral gastroenteritis. Although it can be replicated in\u00a0vitro using human intestinal enteroids (HIEs), generating high\u2010titer viral laboratory stocks remains challenging due to low infection and passage efficiencies. Interferon response and chemokine signaling may be key host pathways that restrict efficient HuNoV replication. Here, we evaluated the effect of their inhibitors, ruxolitinib\u2014a Janus kinase (JAK) 1\/JAK2 inhibitor\u2014and TAK\u2010779\u2014a C\u2010X\u2010C motif chemokine receptor 3 (CXCR3)\/C\u2010C motif chemokine receptor (CCR) 5\/CCR2 antagonist\u2014on HuNoV replication in wild\u2010type jejunal HIEs. TAK\u2010779 increased GII.17 HuNoV replication by 2.3\u2010 and 6.0\u2010fold at 48 and 96\u2009h postinfection (hpi), respectively, compared with the DMSO\u2010treated control. In contrast, ruxolitinib alone did not affect viral replication. Notably, simultaneous treatment with these compounds further enhanced GII.17 replication by 6.0\u2010 and 10.7\u2010fold at 48 and 96 hpi, respectively, which enabled serial passaging. These findings imply that the simultaneous suppression of these pathways potentially benefits GII.17 HuNoV replication.<\/jats:p>","DOI":"10.1111\/gtc.70139","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T09:45:48Z","timestamp":1785577548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Treatment With Ruxolitinib and\n                    <scp>TAK<\/scp>\n                    \u2010779 Enhances\n                    <scp>GII<\/scp>\n                    .17 Human Norovirus Replication and Enables Serial Passaging in Human Intestinal Enteroids"],"prefix":"10.1111","volume":"31","author":[{"given":"Eri","family":"Hiraishi","sequence":"first","affiliation":[{"name":"Department of Diagnostic Testing and Technology Research National Institute of Infectious Diseases, Japan Institute for Health Security  Tokyo Japan"},{"name":"Department of Health Science Gunma Paz University Graduate School of Health Sciences  Takasaki Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Virology II National Institute of Infectious Diseases, Japan Institute for Health Security  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4519-0309","authenticated-orcid":false,"given":"Yoshiki","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Virology II National Institute of Infectious Diseases, Japan Institute for Health Security  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Health Science Gunma Paz University Graduate School of Health Sciences  Takasaki Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1712-7433","authenticated-orcid":false,"given":"Kosuke","family":"Murakami","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Testing and Technology Research National Institute of Infectious Diseases, Japan Institute for Health Security  Tokyo Japan"},{"name":"Department of Virology II National Institute of Infectious Diseases, Japan Institute for Health Security  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201036398\u2010z"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3107.250524"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2308.161138"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001318"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2408.180126"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.39494"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.15479"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5211"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00448\u201024"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/gtc.70007"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00623\u201021"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00215\u201020"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.41.4.1548\u20101557.2003"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aeb0455"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2526726122"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2010834117"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.11.005"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00855\u201022"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1910138117"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2018.11.001"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13030461"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2012.11.011"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(18)30059\u20108"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008009"},{"key":"e_1_2_11_26_1","volume-title":"WHO 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