{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:12:28Z","timestamp":1777594348247,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,6,4]],"date-time":"2014-06-04T00:00:00Z","timestamp":1401840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000784","name":"European Parliament","doi-asserted-by":"publisher","award":["226956"],"award-info":[{"award-number":["226956"]}],"id":[{"id":"10.13039\/501100000784","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Groundwater"],"published-print":{"date-parts":[[2015,3]]},"abstract":"Abstract<\/jats:title>\n \n In situ chemical oxidation (\n ISCO<\/jats:styled-content>\n ) followed by a bioremediation step is increasingly being considered as an effective biphasic technology. Information on the impact of chemical oxidants on organohalide respiring bacteria (\n OHRB<\/jats:styled-content>\n ), however, is largely lacking. Therefore, we used quantitative\n PCR<\/jats:styled-content>\n (\n qPCR<\/jats:styled-content>\n ) to monitor the abundance of\n OHRB<\/jats:styled-content>\n (\n Dehalococcoides mccartyi<\/jats:italic>\n ,\n Dehalobacter<\/jats:italic>\n ,\n Geobacter<\/jats:italic>\n , and\n Desulfitobacterium<\/jats:italic>\n ) and reductive dehalogenase genes (\n rdh<\/jats:italic>\n ;\n \n tceA<\/jats:styled-content>\n <\/jats:italic>\n ,\n \n vcrA<\/jats:styled-content>\n <\/jats:italic>\n , and\n \n bvcA<\/jats:styled-content>\n <\/jats:italic>\n ) at a field location contaminated with chlorinated solvents prior to and following treatment with sodium persulfate. Natural attenuation of the contaminants tetrachloroethene (\n PCE<\/jats:styled-content>\n ) and trichloroethene (\n TCE<\/jats:styled-content>\n ) observed prior to\n ISCO<\/jats:styled-content>\n was confirmed by the distribution of\n OHRB<\/jats:styled-content>\n and\n rdh<\/jats:italic>\n genes. In wells impacted by persulfate treatment, a 1 to 3 order of magnitude reduction in the abundances of\n OHRB<\/jats:styled-content>\n and complete absence of\n rdh<\/jats:italic>\n genes was observed 21 days after\n ISCO<\/jats:styled-content>\n . Groundwater acidification (\n pH<\/jats:styled-content>\n <3) and increase in the oxidation reduction potential (>500\n mV<\/jats:styled-content>\n ) due to persulfate treatment were significant and contributed to disruption of the microbial community. In wells only mildly impacted by persulfate, a slight stimulation of the microbial community was observed, with more than 1 order of magnitude increase in the abundance of\n Geobacter<\/jats:italic>\n and\n Desulfitobacterium<\/jats:italic>\n 36 days after\n ISCO<\/jats:styled-content>\n . After six months, regeneration of the\n OHRB<\/jats:styled-content>\n community occurred, however, neither\n D. mccartyi<\/jats:italic>\n nor any\n rdh<\/jats:italic>\n genes were observed, indicating extended disruption of biological natural attenuation (\n NA<\/jats:styled-content>\n ) capacity following persulfate treatment. For full restoration of biological\n NA<\/jats:styled-content>\n activity, additional time may prove sufficient; otherwise addition electron donor amendment or bioaugmentation may be required.\n <\/jats:p>","DOI":"10.1111\/gwat.12209","type":"journal-article","created":{"date-parts":[[2014,6,4]],"date-time":"2014-06-04T15:37:52Z","timestamp":1401896272000},"page":"261-270","source":"Crossref","is-referenced-by-count":20,"title":["Microbial Dynamics During and After In Situ Chemical Oxidation of Chlorinated Solvents"],"prefix":"10.1111","volume":"53","author":[{"given":"Nora B.","family":"Sutton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siavash","family":"Atashgahi","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University Dreijenplein 10, 6703 HB Wageningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jurgen","family":"van der Wal","sequence":"additional","affiliation":[{"name":"Arcadis, Apeldoorn het Rietveld 59a, 7321 CT Apeldoorn The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert","family":"Wijn","sequence":"additional","affiliation":[{"name":"Arcadis, Apeldoorn het Rietveld 59a, 7321 CT Apeldoorn The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Grotenhuis","sequence":"additional","affiliation":[{"name":"Environmental Technology Wageningen University Bornse Weilanden 9, 6708 WG Wageningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hauke","family":"Smidt","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University Dreijenplein 10, 6703 HB Wageningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huub H.M.","family":"Rijnaarts","sequence":"additional","affiliation":[{"name":"Environmental Technology Wageningen University Bornse Weilanden 9, 6708 WG Wageningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12044"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconhyd.2010.02.004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2461-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/es803308q"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2013.09.003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2009.00820.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1029\/2007WR006633"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/es049017y"},{"key":"e_1_2_6_10_1","unstructured":"ISO.2008. 10301: Water quality: Determination of highly volatile halogenated hydrocarbons\u2014gas chromatographic methods. p. 42."},{"key":"e_1_2_6_11_1","unstructured":"ISO.2002. 10381\u20132: Soil quality sampling: Guidance on sampling techniques."},{"key":"e_1_2_6_12_1","unstructured":"ITRC.2005. 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