{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:12:23Z","timestamp":1771236743903,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T00:00:00Z","timestamp":1349913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2013,3]]},"abstract":"Summary<\/jats:title>Effects of desmopressin (DDAVP<\/jats:styled-content>) in platelet disorders and primary haemostasis cannot be attributed solely to the increase in FVIII<\/jats:styled-content>\/VWF<\/jats:styled-content> (von Willebrand factor), as VWF<\/jats:styled-content>\/FVIII<\/jats:styled-content> concentrates have no effect in these circumstances. Microparticles (MP<\/jats:styled-content>) can support haemostasis by expression of phospholipids, tissue factor and VWF<\/jats:styled-content> on their surface. We hypothesized that significant amounts of VWF<\/jats:styled-content> are bound to MP<\/jats:styled-content> after DDAVP<\/jats:styled-content> administration and that consequently depletion of MP<\/jats:styled-content> should influence VWF<\/jats:styled-content>:Ag and VWF<\/jats:styled-content>:RC<\/jats:styled-content>o plasma levels. Platelet\u2010poor plasma was either obtained well from healthy controls or before and after DDAVP<\/jats:styled-content> administration from patients with von Willebrand's disease (type 1 or possible type 1) or patients with other bleeding disorders as controls. Concentrations of MP<\/jats:styled-content> and VWF<\/jats:styled-content> parameters were determined before and after MP<\/jats:styled-content> depletion by different methods (magnetic bead selection, plasma microfiltration, ultracentrifugation). Platelet MP<\/jats:styled-content> and VWF<\/jats:styled-content>\u2010bearing MP<\/jats:styled-content> were significantly increased after DDAVP<\/jats:styled-content>. MP<\/jats:styled-content> depletion by magnetic bead selection led to a significant reduction in VWF<\/jats:styled-content>:Ag (\u221218.0%) and VWF<\/jats:styled-content>:RC<\/jats:styled-content>o (\u221227.7%) plasma levels without changes in VWF<\/jats:styled-content> multimer composition. As results were similar for DDAVP<\/jats:styled-content> control subjects, the amount of VWF<\/jats:styled-content> bound to circulating microparticles was significantly higher after DDAVP<\/jats:styled-content> administration compared with healthy controls (reduction \u221211.7%). DDAVP<\/jats:styled-content> leads to a release of microparticles and increases the amount of VWF<\/jats:styled-content> bound to microparticles which might explain the clinical efficacy of DDAVP<\/jats:styled-content> in platelet disorders.<\/jats:p>","DOI":"10.1111\/hae.12032","type":"journal-article","created":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T14:02:40Z","timestamp":1349964160000},"page":"236-241","source":"Crossref","is-referenced-by-count":9,"title":["Increased amounts of von Willebrand factor are bound to microparticles after infusion of desmopressin"],"prefix":"10.1111","volume":"19","author":[{"given":"A.","family":"Trummer","sequence":"first","affiliation":[{"name":"Department of Haematology, Haemostasis, Oncology and Stem Cell Transplantation Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Haarmeijer","sequence":"additional","affiliation":[{"name":"Department of Haematology, Haemostasis, Oncology and Stem Cell Transplantation Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Werwitzke","sequence":"additional","affiliation":[{"name":"Department of Haematology, Haemostasis, Oncology and Stem Cell Transplantation Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Wermes","sequence":"additional","affiliation":[{"name":"Department of Haematology, Haemostasis, Oncology and Stem Cell Transplantation Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ganser","sequence":"additional","affiliation":[{"name":"Department of Haematology, Haemostasis, Oncology and Stem Cell Transplantation Hannover Medical School Hannover 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