{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T20:39:08Z","timestamp":1780432748881,"version":"3.54.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T00:00:00Z","timestamp":1418860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of this study was to evaluate the capability of thromboelastometry (ROTEM) and thrombin generation assay (TGA) to monitor the treatment response of bypassing agent (BPA) therapy and to study whether one method is superior to another. In a prospective crossover study haemophilia A patients with high titre inhibitors were included to receive a dose of 75\u00a0U\u00a0kg<jats:sup>\u22121<\/jats:sup> activated prothrombin complex concentrates (<jats:styled-content style=\"fixed-case\">aPCC<\/jats:styled-content>) intravenously. Blood sampling was performed at baseline, 15, 30\u00a0min, 1, 2, 3 and 4\u00a0h post\u2010infusion for TGA and ROTEM analysis. After a washout period of 14\u00a0days the subjects received recombinant FVIIa (<jats:styled-content style=\"fixed-case\">rFVII<\/jats:styled-content>a) at a dose of 90\u00a0\u03bcg\u00a0kg<jats:sup>\u22121<\/jats:sup> and similar blood sampling was performed. Healthy subjects were used as controls. Six haemophilia A patients with inhibitors were included. We found that TGA parameters endogenous thrombin potential (ETP) and peak thrombin increased 2\u20133 folds from baseline 15\u201330\u00a0min after infusion. ROTEM parameters MaxVel and maximum clot firmness increased to a level comparable to that of healthy controls. An individual difference in response was observed for different parameters among participants. ETP and peak thrombin were almost two\u2010fold greater following <jats:styled-content style=\"fixed-case\">aPCC<\/jats:styled-content> infusion compared to <jats:styled-content style=\"fixed-case\">rFVII<\/jats:styled-content>a, whereas ROTEM parameters showed no difference in response between the two products. The study showed that ROTEM and TGA have a great potential to evaluate the effect of BPA in haemophilia patients with inhibitors. TGA seemed to be more sensitive than ROTEM in reflecting the difference in treatment response between <jats:styled-content style=\"fixed-case\">aPCC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">rFVII<\/jats:styled-content>a. Additional prospective clinical studies are needed to clarify which assay and what parameters are clinically predictive.<\/jats:p>","DOI":"10.1111\/hae.12570","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:54:28Z","timestamp":1418972068000},"page":"275-283","source":"Crossref","is-referenced-by-count":37,"title":["Monitoring bypassing agent therapy \u2013 a prospective crossover study comparing thromboelastometry and thrombin generation assay"],"prefix":"10.1111","volume":"21","author":[{"given":"H. T. 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