{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:28:10Z","timestamp":1789810090933,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T00:00:00Z","timestamp":1425945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The platelet function analyser (<jats:styled-content style=\"fixed-case\">PFA<\/jats:styled-content>\u2010100) is a biological tool designed to explore primary haemostasis. This system has thus been widely demonstrated as reliable in detecting von Willebrand factor (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>) deficiency. However, most studies were based on patients benefitting from regular medical care and accurate diagnosis, and it would seem probable that the results were somewhat optimistic, and do not reflect its performances in \u2018real\u2010world\u2019 situations. We have chosen to study the reliability of <jats:styled-content style=\"fixed-case\">PFA<\/jats:styled-content>\u2010100 for screening <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> ristocetin cofactor (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RCo<\/jats:styled-content>) deficiency. We retrospectively analysed the results (<jats:italic>n<\/jats:italic>\u00a0=\u00a06431) of 4027 patients referred to our centre between October 1997 and June 2013 and in whom <jats:styled-content style=\"fixed-case\">PFA<\/jats:styled-content>\u2010Epi, <jats:styled-content style=\"fixed-case\">PFA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:RCo activity had been evaluated. We studied the influence of blood group on the results and the performances of each method in a subgroup of 213 patients with genetically confirmed von Willebrand disease. We have shown that the <jats:styled-content style=\"fixed-case\">PFA<\/jats:styled-content>\u2010100 system, in our experience, constitutes an excellent screening test for detecting <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:RCo deficiency, whatever the clinical situation, in \u2018real\u2010world\u2019 conditions. The negative predictive value (<jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content>), the positive predictive value, the sensitivity and the specificity were respectively: 0.98, 0.51, 0.98 and 0.40. When values adjusted for blood group are used, <jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content> and sensitivity are inferior to those using normal values which have not been adjusted for blood group. We have shown the <jats:styled-content style=\"fixed-case\">PFA<\/jats:styled-content>\u2010100 method to be more efficient in screening for <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> deficiency than the <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:RCo technique.<\/jats:p>","DOI":"10.1111\/hae.12653","type":"journal-article","created":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T07:10:47Z","timestamp":1425971447000},"page":"646-652","source":"Crossref","is-referenced-by-count":49,"title":["Platelet function analyser (<scp>PFA<\/scp>\u2010100) results and von Willebrand factor deficiency: a 16\u2010year \u2018real\u2010world\u2019 experience"],"prefix":"10.1111","volume":"21","author":[{"given":"L.","family":"Ardillon","sequence":"first","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Ternisien","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Fouassier","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Sigaud","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lefran\u00e7ois","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Pacault","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Ribeyrol","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Fressinaud","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Boisseau","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Trossa\u00ebrt","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and European Haemophilia Comprehensive Care Centre University Hospital Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1613992"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1334867"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651343"},{"key":"e_1_2_8_5_1","first-page":"178","article-title":"Lower limit of assay sensitivity: an under\u2010recognised and significant problem in von Willebrand disease identification and classification","volume":"21","author":"Favaloro EJ","year":"2008","journal-title":"Clin Lab 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