{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T23:29:58Z","timestamp":1723850998592},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T00:00:00Z","timestamp":1460592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Diagnosis of von Willebrand disease (<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>) requires quantitative as well as qualitative determination of von Willebrand factor (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>) levels. For functional assessment of <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>, ristocetin cofactor assay by aggregometry is considered to be the gold standard. However, need for technical expertise, labour intensiveness, difficult standardization and high intra\u2010 and inter\u2010 assay variabilities are some of the limitations of this methodology. Various assays for determination of <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> adhesive function using different methodologies have been developed in recent years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the Hemos<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> AcuStar chemiluminescence assay (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Acu]) and the Hemos<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> latex immunoassay (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:act) as diagnostic tests for <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> and identification of type 2 <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> in comparison with the ristocetin cofactor assay performed by aggregometry (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Agg]).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Results from 96 samples analysed by <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Acu] and 128 samples by <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:act were compared with <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Agg]. Sixty of these samples (25 normal, 17 type 1 and 18 type 2) were analysed by all three assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Acu] showed excellent agreement with <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Agg], and readily identified all type 2 <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> samples tested. <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:act showed reasonable agreement with <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Agg] for most patients, but had a slightly lower sensitivity for detection of type 2 <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Acu] assay has the potential to replace <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>:<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>o[Agg] for the diagnosis of <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.12937","type":"journal-article","created":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T17:31:14Z","timestamp":1460655074000},"source":"Crossref","is-referenced-by-count":10,"title":["Comparison of von Willebrand factor (<scp>VWF<\/scp>) activity levels determined by Hemos<scp>IL<\/scp> AcuStar assay and Hemos<scp>IL LIA<\/scp> assay with ristocetin cofactor assay by aggregometry"],"prefix":"10.1111","volume":"22","author":[{"given":"S.","family":"Sagheer","sequence":"first","affiliation":[{"name":"Haematology Division SA Pathology Adelaide Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Rodgers","sequence":"additional","affiliation":[{"name":"Haematology Division SA Pathology Adelaide Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Yacoub","sequence":"additional","affiliation":[{"name":"Haematology Division SA Pathology Adelaide Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Dauer","sequence":"additional","affiliation":[{"name":"Haematology Department The Alfred Prahan Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Mcrae","sequence":"additional","affiliation":[{"name":"Haematology Division SA Pathology Adelaide Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Duncan","sequence":"additional","affiliation":[{"name":"Haematology Division SA Pathology Adelaide Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.2.454.454"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2011.02662.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MBC.0b013e3283367917"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12220"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/00313029700169365"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12862"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2014.05.020"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mbc.0000233365.10981.08"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH14-02-0108"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2010.11.029"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2010.02419.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029606291428"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651343"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1281040"},{"key":"e_1_2_8_16_1","first-page":"163","volume-title":"Basic Method Validation","author":"Barry PL","year":"2003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12203"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12264"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH12-05-0294"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2008.02845.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12064"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12218"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24032"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12087"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12380"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10766"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029610379848"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12443"}],"container-title":["Haemophilia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.12937","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hae.12937","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/hae.12937","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hae.12937","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T16:56:45Z","timestamp":1693414605000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hae.12937"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,14]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1111\/hae.12937"],"URL":"https:\/\/doi.org\/10.1111\/hae.12937","archive":["Portico"],"relation":{},"ISSN":["1351-8216","1365-2516"],"issn-type":[{"value":"1351-8216","type":"print"},{"value":"1365-2516","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,14]]}}}