{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:33:33Z","timestamp":1785929613037,"version":"3.56.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T00:00:00Z","timestamp":1464048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Although the variability in factor VIII (<jats:styled-content style=\"fixed-case\">FVIII<\/jats:styled-content>):C measurement is well recognized, this has not been widely reported for post\u2010<jats:styled-content style=\"fixed-case\">FVIII<\/jats:styled-content> infusion samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim\/Methods<\/jats:title><jats:p>Three samples from haemophilia A patients were distributed in a <jats:styled-content style=\"fixed-case\">UK<\/jats:styled-content> National External Quality Assessment Scheme survey, each after treatment with either ReFacto <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content>, Kogenate <jats:styled-content style=\"fixed-case\">FS<\/jats:styled-content> or Advate. Fifty\u2010two <jats:styled-content style=\"fixed-case\">UK<\/jats:styled-content> haemophilia centres performed <jats:styled-content style=\"fixed-case\">FVIII<\/jats:styled-content> assays using one\u2010stage (<jats:italic>n<\/jats:italic> = 46) and chromogenic (<jats:italic>n<\/jats:italic> = 10) assays. Centres calibrated assays with the local plasma standard and with ReFacto <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content> laboratory standard for the ReFacto <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content> sample.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results\/Conclusions<\/jats:title><jats:p>Chromogenic assays gave significantly higher results than one\u2010stage assays (<jats:italic>P<\/jats:italic> &lt; 0.0001, 32% difference) in the post\u2010Kogenate sample but not in the post\u2010ReFacto <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content> (11% higher by chromogenic assay, ns) or post\u2010Advate samples (3% lower by chromogenic, ns) when assays were calibrated with plasma standards. Twenty centres used all Instrumentation Laboratory (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u2010activated partial thromboplastin time reagents (Synthasil)\/<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> deficient plasma\/reference plasma) in the one\u2010stage assay and 15 used all Siemens reagents (Actin <jats:styled-content style=\"fixed-case\">FS<\/jats:styled-content>\/Siemens deficient plasma\/reference plasma); this made a significant difference to results post\u2010ReFacto <jats:styled-content style=\"fixed-case\">AF<\/jats:styled-content> (41% higher by <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> reagents, <jats:italic>P<\/jats:italic> &lt; 0.0001) and Advate (39% higher by <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> reagents, <jats:italic>P<\/jats:italic> &lt; 0.0001), but not Kogenate (7% higher by <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>, ns) when calibrated with plasma standards. Differences between results obtained with different one\u2010stage assay reagents for monitoring Advate have implications for dosing patients. Furthermore, there was considerable inter\u2010laboratory variation as indicated by <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>s in the range 15\u201326% for chromogenic assay and 12\u201319% for one\u2010stage assay results. This study suggests that external quality assessment schemes should offer participation in post\u2010<jats:styled-content style=\"fixed-case\">FVIII<\/jats:styled-content> infusion schemes where haemophilic patients are monitored.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.12962","type":"journal-article","created":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T19:43:26Z","timestamp":1464119006000},"page":"806-812","source":"Crossref","is-referenced-by-count":17,"title":["Factor <scp>VIII<\/scp> assay variability in postinfusion samples containing full length and B\u2010domain deleted <scp>FVIII<\/scp>"],"prefix":"10.1111","volume":"22","author":[{"given":"S.","family":"Kitchen","sequence":"first","affiliation":[{"name":"UK NEQAS Blood Coagulation Sheffield UK"},{"name":"Sheffield Haemophilia and Thrombosis Centre Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Jennings","sequence":"additional","affiliation":[{"name":"UK NEQAS Blood Coagulation Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Makris","sequence":"additional","affiliation":[{"name":"UK NEQAS Blood Coagulation Sheffield UK"},{"name":"Department of Cardiovascular Science University of Sheffield Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. P.","family":"Kitchen","sequence":"additional","affiliation":[{"name":"UK NEQAS Blood Coagulation Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. A. L.","family":"Woods","sequence":"additional","affiliation":[{"name":"UK NEQAS Blood Coagulation Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. 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