{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T11:30:33Z","timestamp":1787398233573,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,6,13]],"date-time":"2016-06-13T00:00:00Z","timestamp":1465776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministero dell'Universit\u00e0 e della Ricerca Scientifica e Tecnologica"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The heterogeneity of von Willebrand disease (<jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>) makes its diagnosis a difficult task.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We report here on the usefulness of a microchip\u2010based flow\u2010chamber system, the total thrombus\u2010formation analysis system (T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content>), in the identification and characterization of <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>. Thirty <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> patients and 20 healthy subjects were studied with the T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content> platelet (<jats:styled-content style=\"fixed-case\">PL<\/jats:styled-content>) and atherome (<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>) microchips developed for the <jats:italic>in vitro<\/jats:italic> assessment of platelet thrombus formation and fibrin\u2010rich platelet thrombus formation respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Samples from severe type 1 <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content>, characterized by von Willebrand factor (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content>) levels below 10 U dL<jats:sup>\u22121<\/jats:sup>, failed to occlude either the <jats:styled-content style=\"fixed-case\">PL<\/jats:styled-content> or the <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> chip capillaries, while the occlusion times were normal in patients with mild type 1 <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> above 25 U dL<jats:sup>\u22121<\/jats:sup>). <jats:styled-content style=\"fixed-case\">PL<\/jats:styled-content> and\/or <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> chip occlusion occurred, but took longer than normal, for samples from type Vicenza and type 1 <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> patients, whose <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> levels ranged between 10 and 25 U dL<jats:sup>\u22121<\/jats:sup>. No <jats:styled-content style=\"fixed-case\">PL<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> chip capillary occlusion was seen for samples from patients with type 2A or 2B <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> featuring the absence of large <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> multimers, whereas no abnormalities emerged for type 2B patients with normal multimer patterns.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content> appears to be sensitive mainly to plasma <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> concentration and the presence of large multimers. Failure of the <jats:styled-content style=\"fixed-case\">PL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> chips to become occluded points to a lack of large <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> multimers, or type 1 <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">VWF<\/jats:styled-content> levels below 10 U dL<jats:sup>\u22121<\/jats:sup>. Although the T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content> does not assure a precise <jats:styled-content style=\"fixed-case\">VWD<\/jats:styled-content> diagnosis, it does point us in the right direction, and thus seems a useful global preliminary test.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.12971","type":"journal-article","created":{"date-parts":[[2016,6,13]],"date-time":"2016-06-13T10:09:38Z","timestamp":1465812578000},"page":"949-956","source":"Crossref","is-referenced-by-count":23,"title":["Usefulness of the Total Thrombus\u2010Formation Analysis System (T\u2010<scp>TAS<\/scp>) in the diagnosis and characterization of von Willebrand disease"],"prefix":"10.1111","volume":"22","author":[{"given":"V.","family":"Daidone","sequence":"first","affiliation":[{"name":"Department of Cardiologic, Thoracic and Vascular Sciences Thrombohemorrhagic Disorders Unit University of Padua Medical School Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Barbon","sequence":"additional","affiliation":[{"name":"Veneto Institute of Oncology IRCCS Familial Cancer Clinic and Oncoendocrinology Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Casonato","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00062752-200303000-00008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.395"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47430-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.4.895.895"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.56.082103.104713"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02146.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-122945"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.00975.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1182\/blood.V69.6.1691.1691","article-title":"The effect of ABO blood group in the diagnosis of von Willebrand disease","volume":"69","author":"Gill JC","year":"1987","journal-title":"Blood"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02790.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(89)90317-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1055\/s-0038-1661167","article-title":"Hemostasis in normal pregnancy","volume":"52","author":"Stirling Y","year":"1984","journal-title":"Thromb Haemost"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12664"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2009.12.003"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.1.180"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199904000-00006"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2010.03.003"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04464.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2011.11.007"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2012.02867.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2013.05.026"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH07-05-0347"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1182\/blood.V58.2.387.387","article-title":"Effect of multimeric structure of the factor VIII\/von Willebrand factor protein on binding platelets","volume":"58","author":"Gralnick HR","year":"1981","journal-title":"Blood"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH10-10-0634"}],"container-title":["Haemophilia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.12971","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.12971","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hae.12971","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T20:26:29Z","timestamp":1695500789000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hae.12971"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,13]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/hae.12971"],"URL":"https:\/\/doi.org\/10.1111\/hae.12971","archive":["Portico"],"relation":{},"ISSN":["1351-8216","1365-2516"],"issn-type":[{"value":"1351-8216","type":"print"},{"value":"1365-2516","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,13]]}}}