{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T03:55:33Z","timestamp":1772078133531,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,6,28]],"date-time":"2016-06-28T00:00:00Z","timestamp":1467072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Haemophilia is a lifelong X\u2010linked recessive inherited bleeding disorder. Since the haemophilia management in economically less\u2010developed countries is inadequately provided, prevention of new cases of haemophilia is essentially required.<\/jats:p><\/jats:sec><jats:sec><jats:title>Subjects and methods<\/jats:title><jats:p>A total of 42 pregnancies in 37 women at risk for severe and moderate haemophilia (A = 33, B = 4) were enrolled. The prenatal diagnostic (<jats:styled-content style=\"fixed-case\">PND<\/jats:styled-content>) procedure was performed in 32 women, while 10 women refused further <jats:styled-content style=\"fixed-case\">PND<\/jats:styled-content> procedure after knowing their foetuses were female (<jats:italic>n<\/jats:italic> = 8) and male (<jats:italic>n<\/jats:italic> = 2). The foetal specimen was obtained through chorionic villus sampling (<jats:italic>n<\/jats:italic> = 14), amniocentesis (<jats:italic>n<\/jats:italic> = 1) and cordocentesis (<jats:italic>n<\/jats:italic> = 17). The status of haemophilia was determined using informative <jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> markers and inversion of intron 22 of the <jats:italic>F8<\/jats:italic> gene, and\/or foetal <jats:styled-content style=\"fixed-case\">FVIII<\/jats:styled-content>:C or <jats:styled-content style=\"fixed-case\">FIX<\/jats:styled-content>:C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The final diagnosis revealed normal males (<jats:italic>n<\/jats:italic> = 18), haemophilia A males (<jats:italic>n<\/jats:italic> = 9), normal females (<jats:italic>n<\/jats:italic> = 3) and haemophilia A carrier females (<jats:italic>n<\/jats:italic> = 2). All women with affected haemophilia sons requested to terminate their pregnancies except one woman. One of 32 pregnancies (3.1%) had spontaneous abortion. At follow\u2010up after birth, the <jats:styled-content style=\"fixed-case\">PND<\/jats:styled-content> was accurately confirmed in one haemophilia A male, three normal females and two carrier females by laboratory testing, and 18 unaffected normal males by history taking of no bleeding manifestations. However, 10 women who continued their pregnancies after knowing foetal sex turned out to have two haemophilia A males, one normal female, one haemophilia A carrier female and six normal or carrier females.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">PND<\/jats:styled-content> of haemophilia could be accurately determined but it was not well accepted by all couples at risk.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.13002","type":"journal-article","created":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T16:01:24Z","timestamp":1467216084000},"page":"880-885","source":"Crossref","is-referenced-by-count":7,"title":["Prenatal diagnosis for haemophilia: the Thai experience"],"prefix":"10.1111","volume":"22","author":[{"given":"A.","family":"Chuansumrit","sequence":"first","affiliation":[{"name":"Department of Paediatrics Ramathibodi Hospital Mahidol University Bangkok 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