{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:02:20Z","timestamp":1761541340126},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,9,9]],"date-time":"2016-09-09T00:00:00Z","timestamp":1473379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004191","name":"Novo Nordisk","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004191","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001734","name":"K\u00f8benhavns Universitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001734","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>A major complication of haemophilia is haemophilic arthropathy (HA), a debilitating disorder with an incompletely defined pathobiology. High\u2010resolution imaging may provide new knowledge about onset and progression of HA, and thereby support identification of new treatment opportunities. Recently, a F8<jats:sup>\u2212\/\u2212<\/jats:sup> rat model of HA was developed. The size of the rat allows for convenient and high resolution imaging of the joints, which could enable <jats:italic>in vivo<\/jats:italic> studies of HA development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To determine whether HA in the F8<jats:sup>\u2212\/\u2212<\/jats:sup> rat can be visualized using ultrasonography (US) and micro\u2010computed tomography (\u03bcCT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty F8<jats:sup>\u2212\/\u2212<\/jats:sup> and 20 wild\u2010type rats were subjected to a single or two induced knee bleeds. F8<jats:sup>\u2212\/\u2212<\/jats:sup> rats were treated with either recombinant human FVIII (rhFVIII) or vehicle before the induction of knee bleeds. Haemophilic arthropathy was visualized using <jats:italic>in vivo<\/jats:italic> US and <jats:italic>ex vivo<\/jats:italic> \u03bcCT, and the observations correlated with histological evaluation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>US and \u03bcCT detected pathologies in the knee related to HA. There was a strong correlation between disease severity determined by \u03bcCT and histopathology. rhFVIII treatment reduced the pathology identified with both imaging techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>US and \u03bcCT are suitable imaging techniques for detection of blood\u2010induced joint disease in F8<jats:sup>\u2212\/\u2212<\/jats:sup> rats and may be used for longitudinal studies of disease progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.13080","type":"journal-article","created":{"date-parts":[[2016,9,9]],"date-time":"2016-09-09T17:51:19Z","timestamp":1473443479000},"page":"152-162","source":"Crossref","is-referenced-by-count":9,"title":["Visualization of haemophilic arthropathy in F8<sup>\u2212\/\u2212<\/sup> rats by ultrasonography and micro\u2010computed tomography"],"prefix":"10.1111","volume":"23","author":[{"given":"K. R.","family":"Christensen","sequence":"first","affiliation":[{"name":"Translational Haemophilia Pharmacology Novo Nordisk A\/S Maaloev Denmark"},{"name":"Veterinary Disease Biology University of Copenhagen Frederiksberg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Roepstorff","sequence":"additional","affiliation":[{"name":"Histology &amp; Bioimaging Novo Nordisk A\/S Maaloev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Petersen","sequence":"additional","affiliation":[{"name":"Histology &amp; Bioimaging Novo Nordisk A\/S Maaloev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Wiinberg","sequence":"additional","affiliation":[{"name":"Translational Haemophilia Pharmacology Novo Nordisk A\/S Maaloev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. K.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Veterinary Disease Biology University of Copenhagen Frederiksberg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kjelgaard\u2010Hansen","sequence":"additional","affiliation":[{"name":"Translational Haemophilia Pharmacology Novo Nordisk A\/S Maaloev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. N.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Translational Haemophilia Pharmacology Novo Nordisk A\/S Maaloev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13405-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.1994.tb00815.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-01-528414"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12635"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13328"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2010.02472.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2010.02307.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12399"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2009.02044.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12268"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2012.02888.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13259"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-339-8_24"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2012.02909.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2007.083915"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24133"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12743"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12637"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12175"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2011.02672.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2008.01689.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sult.2013.05.007"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2012.02834.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-10-602060"}],"container-title":["Haemophilia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.13080","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.13080","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hae.13080","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:41:07Z","timestamp":1695490867000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hae.13080"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,9]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1111\/hae.13080"],"URL":"https:\/\/doi.org\/10.1111\/hae.13080","archive":["Portico"],"relation":{},"ISSN":["1351-8216","1365-2516"],"issn-type":[{"value":"1351-8216","type":"print"},{"value":"1365-2516","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,9]]}}}