{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T12:38:15Z","timestamp":1771504695123,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T00:00:00Z","timestamp":1493856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["26461593"],"award-info":[{"award-number":["26461593"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["15K09663"],"award-info":[{"award-number":["15K09663"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Red blood cells (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s) contribute to hemostasis under blood\u2010flow, and anemia might contribute to a hemorrhagic diathesis. The majority of current laboratory techniques to assess hemostasis do not consider the effects of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s. An assay to determine the role of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s in hemostasis could be beneficial for clinical management.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the influence of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s in hemostasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hemostasis was investigated using a novel microchip flow\u2010chamber system (T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup>) in an anemic patient with von Willebrand disease. Subsequently, the effects of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s in total thrombus analysis system (T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content>) were examined using reconstituted whole blood at various hematocrit levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>In vivo<\/jats:italic>: When the patient was anemic and demonstrated persisted hemorrhagic symptoms despite the maintained adequate von Willebrand factor ristocetin cofactor activity levels, thrombus formation determined by T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content> was delayed. However, transfusions of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s resolved bleeding symptom and, accordingly, the thrombus formation in T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content> improved. <jats:italic>In vitro<\/jats:italic>: Thrombus formation determined by T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content> at 1000 s<jats:sup>\u22121<\/jats:sup> was dose\u2010dependent on hematocrit (the time to reach 10 kPa (<jats:italic>T<\/jats:italic><jats:sub>10<\/jats:sub>): 10.0 \u00b1 0, 9.5 \u00b1 1.4, 6.7 \u00b1 2.4, 2.8 \u00b1 1.6 min at hematocrits of 0%, 12.5%, 25% and 50%, respectively). Markedly defective thrombus formation (<jats:italic>T<\/jats:italic><jats:sub>10<\/jats:sub> &gt;10 min) was confirmed at a hematocrit &lt;25% at 2000 s<jats:sup>\u22121<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Red blood cells play an essential role in hemostasis under high shear, and <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> transfusions could be effective for refractory bleeding in patients with anemia. T\u2010<jats:styled-content style=\"fixed-case\">TAS<\/jats:styled-content> measurements appear to reflect the hemostatic consequences of diminished red cell numbers under blood\u2010flow, and could provide a valuable means for monitoring patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.13252","type":"journal-article","created":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T06:56:40Z","timestamp":1493881000000},"page":"750-758","source":"Crossref","is-referenced-by-count":16,"title":["Role of red blood cells in the anemia\u2010associated bleeding under high shear conditions"],"prefix":"10.1111","volume":"23","author":[{"given":"H.","family":"Yaoi","sequence":"first","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1067-5979","authenticated-orcid":false,"given":"Y.","family":"Shida","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Ogiwara","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Research Institute Fujimori Kogyo Co., Ltd. Yokohama Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Shima","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nogami","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7352265"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V62.1.214.214"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.8.6.819"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-09-521484"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-09-110700"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1961.tb00318.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38111299056308.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.008417"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038705"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2012.01.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14148"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH09-05-0284"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2013.849804"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1614035"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.4.1325"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04464.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2011.11.007"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086491"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12610"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-015-1819-8"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13273"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2012.02867.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/hq0202.104122"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2007.07.019"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-014-1720-x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12987"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.03975.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00656.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.01771.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2516.2001.00486.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08333.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.8.2939.bloodjournal8882939"}],"container-title":["Haemophilia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.13252","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/chorus\/v1\/articles\/10.1111%2Fhae.13252","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hae.13252","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T14:03:29Z","timestamp":1695564209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hae.13252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,4]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/hae.13252"],"URL":"https:\/\/doi.org\/10.1111\/hae.13252","archive":["Portico"],"relation":{},"ISSN":["1351-8216","1365-2516"],"issn-type":[{"value":"1351-8216","type":"print"},{"value":"1365-2516","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,4]]}}}