{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:16:17Z","timestamp":1740125777696,"version":"3.37.3"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,22]],"date-time":"2017-11-22T00:00:00Z","timestamp":1511308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2018,1]]},"abstract":"Introduction<\/jats:title>Laboratory diagnosis of von Willebrand disease (VWD<\/jats:styled-content>) is made by the measurement of von Willebrand factor (VWF<\/jats:styled-content>) protein level and its activities. Current VWF<\/jats:styled-content> activity tests include ristocetin cofactor and collagen binding (VWF<\/jats:styled-content>:CB<\/jats:styled-content>) assays.<\/jats:p><\/jats:sec>Aim<\/jats:title>We have undertaken an evaluation of a new fully automated VWF<\/jats:styled-content>:CB<\/jats:styled-content> assay relative to an established enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) method.<\/jats:p><\/jats:sec>Methods<\/jats:title>The two analytical systems operate with different detection principles: a chemiluminescent method performed on ACL<\/jats:styled-content> AcuStar Analyzer (the former) and a colorimetric ELISA<\/jats:styled-content> by Asserachrom Stago (the latter) (type III<\/jats:styled-content> collagen from human placenta). The HemosIL<\/jats:styled-content> AcuStar VWF<\/jats:styled-content>:CB<\/jats:styled-content> assay is a chemiluminescent 2\u2010step immunoassay that uses magnetic particles coated with a type III<\/jats:styled-content> collagen triple\u2010helical peptide. VWF<\/jats:styled-content>:CB<\/jats:styled-content> levels were determined in 50 healthy subjects and 100 VWD<\/jats:styled-content> patients (22 type 1, 73 type 2 and 5 type 3).<\/jats:p><\/jats:sec>Results<\/jats:title>Eleven VWD<\/jats:styled-content> samples reported VWF<\/jats:styled-content>:CB<\/jats:styled-content> values below the lower detection limit of one or both methods. The new method showed a good correlation with the ELISA<\/jats:styled-content> method (r<\/jats:italic>\u00a0>\u00a0.9, mean bias 3.85\u00a0IU<\/jats:styled-content>\/dL<\/jats:styled-content>) in both healthy and VWD<\/jats:styled-content> samples. One of 150 samples gave inconsistent results using the two assays, leading to an uncertain diagnosis of VWD<\/jats:styled-content> type 1 (ELISA<\/jats:styled-content> method) or type 2 MCB<\/jats:styled-content> (fully automated method).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The new assay is rapid and simple to use, with its ready\u2010to\u2010use reagent cartridges. This VWF<\/jats:styled-content>:CB<\/jats:styled-content> assay, in addition to the measurement of VWF<\/jats:styled-content>:Ag and VWF<\/jats:styled-content>:RC<\/jats:styled-content>o made on the same platform, gives additional information for the diagnosis of VWD<\/jats:styled-content> in both nonspecialized and reference laboratories.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.13371","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T04:06:46Z","timestamp":1511410006000},"page":"156-161","source":"Crossref","is-referenced-by-count":6,"title":["A comparative evaluation of a new fully automated assay for von Willebrand factor collagen binding activity to an established method"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5214-0580","authenticated-orcid":false,"given":"F.","family":"Stufano","sequence":"first","affiliation":[{"name":"Angelo Bianchi Bonomi Hemophilia and Thrombosis Center Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico and Luigi Villa Foundation Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Baronciani","sequence":"additional","affiliation":[{"name":"Angelo Bianchi Bonomi Hemophilia and Thrombosis Center Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico and Luigi Villa Foundation Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Mane\u2010Padros","sequence":"additional","affiliation":[{"name":"R&D Biokit R&D Llica d'Amunt Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Cozzi","sequence":"additional","affiliation":[{"name":"Angelo Bianchi Bonomi Hemophilia and Thrombosis Center Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico and Luigi Villa Foundation Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Faraudo","sequence":"additional","affiliation":[{"name":"R&D Biokit R&D Llica d'Amunt Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7423-9864","authenticated-orcid":false,"given":"F.","family":"Peyvandi","sequence":"additional","affiliation":[{"name":"Angelo Bianchi Bonomi Hemophilia and Thrombosis Center Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico and Luigi Villa Foundation Milan Italy"},{"name":"Department of Pathophysiology and Transplantation Universit\u00e0 degli Studi di Milano Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03661.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1538-7836.2003.00260.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107465"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02146.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12964"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651343"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2012.199000"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24556"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2011.02676.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01311.x"},{"key":"e_1_2_8_12_1","first-page":"77","article-title":"A sensitive ristocetin co\u2010factor activity assay with recombinant glycoprotein Ibalpha for the diagnosis of patients with low von Willebrand factor levels","volume":"89","author":"Federici AB","year":"2004","journal-title":"Haematologica"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1614063"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2015.12.010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1616142"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-10-184317"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04702.x"}],"container-title":["Haemophilia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.13371","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hae.13371","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:16:56Z","timestamp":1695489416000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hae.13371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,22]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1111\/hae.13371"],"URL":"https:\/\/doi.org\/10.1111\/hae.13371","archive":["Portico"],"relation":{},"ISSN":["1351-8216","1365-2516"],"issn-type":[{"type":"print","value":"1351-8216"},{"type":"electronic","value":"1365-2516"}],"subject":[],"published":{"date-parts":[[2017,11,22]]}}}