{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:29:27Z","timestamp":1777631367547,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T00:00:00Z","timestamp":1531440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Accurate diagnosis of an inhibitor, a neutralizing antibody to infused factor VIII (FVIII), is essential for appropriate management of haemophilia A (HA). Low\u2010titre inhibitors may be difficult to diagnose due to high rates of false\u2010positive inhibitor results in that range. Transient low\u2010titre inhibitors and false\u2010positive inhibitors may be due to the presence of a lupus anticoagulant (LA) or other non\u2010specific antibodies. Fluorescence immunoassay (FLI) to detect antibodies to FVIII is a sensitive method to identify inhibitors in HA. Evaluations of antibody profiles by various groups have demonstrated that haemophilic inhibitors detected by Nijmegen\u2010Bethesda (NBA) and chromogenic Bethesda (CBA) assays correlate with positivity for anti\u2010FVIII immunoglobulin (Ig) G1 and G4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study sought to determine whether FLI could distinguish false\u2010positive FVIII inhibitor results related to LAs from clinically relevant FVIII inhibitors in HA patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples from haemophilic and non\u2010haemophilic subjects were tested for LA, specific FVIII inhibitors by NBA and CBA, and anti\u2010FVIII immunoglobulin profiles by FLI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No samples from LA\u2010positive non\u2010haemophilic subjects were positive by FLI for anti\u2010FVIII IgG4. Conversely, 91% of NBA\u2010positive samples from haemophilia subjects were positive for anti\u2010FVIII IgG4. Two of 11 haemophilia subjects had samples negative for anti\u2010FVIII IgG4 and CBA, which likely represented LA rather than FVIII inhibitor presence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Assessment of anti\u2010FVIII profiles along with the CBA may be useful to distinguish a clinically relevant low\u2010titre FVIII inhibitor from a transient LA in HA patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.13565","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T14:26:09Z","timestamp":1531491969000},"page":"807-814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Distinguishing lupus anticoagulants from factor VIII inhibitors in haemophilic and non\u2010haemophilic patients"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9330-7604","authenticated-orcid":false,"given":"A. G.","family":"Rampersad","sequence":"first","affiliation":[{"name":"Indiana Hemophilia &amp; Thrombosis Center  Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3930-4565","authenticated-orcid":false,"given":"B.","family":"Boylan","sequence":"additional","affiliation":[{"name":"Division of Blood Disorders National Center on Birth Defects and Developmental Disabilities Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3989-7973","authenticated-orcid":false,"given":"C. H.","family":"Miller","sequence":"additional","affiliation":[{"name":"Division of Blood Disorders National Center on Birth Defects and Developmental Disabilities Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2821-7159","authenticated-orcid":false,"given":"A.","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Indiana Hemophilia &amp; Thrombosis Center  Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,13]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"223","article-title":"The presence of a circulating anti\u2010coagulant in a male member of a hemophiliac family","volume":"57","author":"Lawrence JS","year":"1941","journal-title":"Trans Am Clin Climatol Assoc"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-06-160432"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12768"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1615621"},{"key":"e_1_2_8_6_1","unstructured":"DiMicheleDM.Inhibitors in hemophilia: a primer.Treatment of Hemophilia Monographs.2008; No.7:1\u20109."},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-577643"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-07-444877"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-09-598268"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00520"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.6.629"},{"key":"e_1_2_8_12_1","first-page":"263","volume-title":"Progress in Thrombosis and Haemostasis","author":"Shapiro AD","year":"1982"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1656029"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12259"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2008.01924.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203308101436"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1649988"},{"key":"e_1_2_8_18_1","first-page":"271","article-title":"A chromogenic substrate method for detecting and titrating anti\u2010factor VIII antibodies in the presence of lupus anticoagulant","volume":"87","author":"Blanco AN","year":"2002","journal-title":"Haematologica"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12302"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPMKP94CODILWS"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24104"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2012.02781.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12467"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07232.x"}],"container-title":["Haemophilia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhae.13565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hae.13565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T16:12:19Z","timestamp":1694880739000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hae.13565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,13]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["10.1111\/hae.13565"],"URL":"https:\/\/doi.org\/10.1111\/hae.13565","archive":["Portico"],"relation":{},"ISSN":["1351-8216","1365-2516"],"issn-type":[{"value":"1351-8216","type":"print"},{"value":"1365-2516","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,13]]},"assertion":[{"value":"2018-06-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}