{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T23:17:32Z","timestamp":1780528652952,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T00:00:00Z","timestamp":1547596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Haemophilic arthropathy is the main morbidity of haemophilia. The individual pathological response to the same number of clinically evident joint bleeds is highly variable; thus, it remains unknown if certain joint bleeding characteristics are critical for the development of arthropathy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To study the relation between bleed volume and subsequent development of arthropathy, we aimed to develop quantitative in vivo imaging of active joint bleeds in a mouse model of haemophilia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Haemophilia A (F8\u2010KO) and wild\u2010type (WT) mice were IV\u2010dosed with a micro\u2010CT blood pool contrast agent prior to an induced knee haemarthrosis or sham procedure. The mice were micro\u2010CT scanned five times the following 2\u00a0days to characterise and quantify the induced haemarthrosis in vivo. On Day 14, the mice were euthanized and pathological changes evaluated by histology and micro\u2010CT. Additionally, bleeding characteristics in vehicle\u2010treated F8\u2010KO mice were compared with those of recombinant FVIII (rFVIII)\u2010treated F8\u2010KO mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>F8\u2010KO mice had a significantly larger bleed volume than WT mice at all scan time points. The bleed volume 12\u00a0hours after induction of haemarthrosis correlated with the subsequent degree of arthropathy. Presence of \u00b5CT\u2010detectable bone pathology was associated with a significantly increased bleed volume among F8\u2010KO mice. rFVIII treatment significantly reduced bleed volume in F8\u2010KO mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Quantitative in vivo contrast\u2010enhanced micro\u2010CT imaging can be used to characterize and quantify joint bleeds in a mouse model of haemophilic arthropathy. The bleed volume correlates with the subsequent degree of arthropathy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.13672","type":"journal-article","created":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T13:32:18Z","timestamp":1547645538000},"page":"324-333","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Bleed volume of experimental knee haemarthrosis correlates with the subsequent degree of haemophilic arthropathy"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6701-2087","authenticated-orcid":false,"given":"K\u00e5re Kryger","family":"V\u00f8ls","sequence":"first","affiliation":[{"name":"Global Drug Discovery Novo Nordisk A\/S Maaloev Denmark"},{"name":"Veterinary and Animal Sciences University of Copenhagen Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads","family":"Kjelgaard\u2010Hansen","sequence":"additional","affiliation":[{"name":"Global Drug Discovery Novo Nordisk A\/S Maaloev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8119-2224","authenticated-orcid":false,"given":"Carsten Dan","family":"Ley","sequence":"additional","affiliation":[{"name":"Global Drug Discovery Novo Nordisk A\/S Maaloev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1575-2507","authenticated-orcid":false,"given":"Axel Kornerup","family":"Hansen","sequence":"additional","affiliation":[{"name":"Veterinary and Animal Sciences University of Copenhagen Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maj","family":"Petersen","sequence":"additional","affiliation":[{"name":"Global Drug Discovery Novo Nordisk A\/S Maaloev 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