{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:01:06Z","timestamp":1750204866839,"version":"3.40.5"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T00:00:00Z","timestamp":1552435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["18K07885"],"award-info":[{"award-number":["18K07885"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The haemorrhagic phenotype in patients with von Willebrand disease (VWD) is heterogeneous, and assays of von Willebrand factor ristocetin cofactor activity (VWF:RCo) do not always reflect clinical severity, especially in those individuals classed as type 1 VWD. Recent studies have shown that whole blood ristocetin\u2010induced platelet agglutination (WB\u2010RIPA) using an easy\u2010to\u2010use analyzer, Multiplate\u00ae platelet impedance technique, could be informative as a diagnostic test in VWD, although inconsistencies were evident in patients with the type 1 disorder, possibly associated with clinical symptoms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the relationship between WB\u2010RIPA, bleeding scores (BS) and VWF\u2010related measurements in type 1 VWD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>WB\u2010RIPA assay using the Multiplate\u00ae was performed using whole blood from 55 patients with type 1 VWD. BS was determined using a standardized questionnaire.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>WB\u2010RIPA values were significantly lower in type 1 VWD than in healthy controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001). Weak correlations were apparent between WB\u2010RIPA and VWF:RCo or VWF antigen (VWF:Ag; <jats:italic>r<\/jats:italic>\u00a0=\u00a00.22 or 0.28, respectively). There were significant differences in VWF:RCo (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.036) and VWF:Ag (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0013) between patients with BS \u22654 (defined as abnormal bleeding tendency) and BS &lt;4 (defined as no abnormal bleeding tendency), respectively. However, no significant difference was observed in WB\u2010RIPA between the BS \u22654 group and BS &lt;4 group. Overall, VWD patients with a WB\u2010RIPA level &gt;70\u00a0U did not seem to have an abnormal bleeding tendency, but low levels of WB\u2010RIPA did not correlate with BS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>WB\u2010RIPA did not reflect clinical severity in type 1 VWD patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.13725","type":"journal-article","created":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T22:23:42Z","timestamp":1552515822000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Whole blood ristocetin\u2010induced platelet impedance aggregometry does not reflect clinical severity in patients with type 1 von Willebrand disease"],"prefix":"10.1111","volume":"25","author":[{"given":"Yuto","family":"Nakajima","sequence":"first","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2415-2194","authenticated-orcid":false,"given":"Keiji","family":"Nogami","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Yada","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara Japan"},{"name":"The Course of Hemophilia Education Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Ogiwara","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University Kashihara Nara 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