{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T02:08:41Z","timestamp":1762999721246,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T00:00:00Z","timestamp":1633478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Haemophilia is a bleeding disorder that occurs due to the deficiency of coagulation factors, and the angiogenesis process is an important process underlying the pathophysiology of haemophilic arthropathy. The role of the new adipocytokine endoglin (ENG) in patients with haemophilia is not yet known.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study is to evaluate the association between ENG protein and angiogenesis\u2010related cytokines in patients with haemophilia for the first time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma protein levels and mRNA expressions of ENG and various angiogenesis\u2010associated cytokines were compared in blood samples collected from 28 patients with haemophilia A or B and 29 healthy volunteers. The relationship between the cytokines and ENG were determined by correlation analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Plasma ENG levels and angiogenic markers were found to be significantly higher in patients with haemophilia compared to controls. Real\u2010time PCR studies showed that mRNA expressions of ENG, vascular endothelial growth factor A, hypoxia\u2010inducible factor A, and prostaglandin E2 increased in patients with haemophilia. Correlation analysis showed a significant positive correlation between ENG and angiopoietin\u20102 levels in the haemophilia group. Besides, a significant decrease in annexin\u2010V binding to platelets in haemophilia patients compared to control was found to be related to the bleeding profiles in the patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study determined that ENG protein may be involved in the formation of angiogenesis in haemophilia patients and its effects may be related to angiogenetic marker angiopoietin\u20102 in this process. Our findings contribute to the literature during the determination of target proteins in haemophilia treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.14430","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T23:40:13Z","timestamp":1633736413000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Increased endoglin levels correlated with angiogenesis\u2010associated angiopoietin\u20102 in haemophilia patients"],"prefix":"10.1111","volume":"27","author":[{"given":"Kubra","family":"Karaman","sequence":"first","affiliation":[{"name":"Ankara University Faculty of Pharmacy Department of Biochemistry  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aybuke","family":"Celik","sequence":"additional","affiliation":[{"name":"Ankara University Faculty of Pharmacy Department of Biochemistry  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selin","family":"Aytac","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Paediatrics Division of Paediatric Haematology Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2809-8946","authenticated-orcid":false,"given":"Filiz","family":"Bakar\u2010Ates","sequence":"additional","affiliation":[{"name":"Ankara University Faculty of Pharmacy Department of Biochemistry  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"107602961989268","DOI":"10.1177\/1076029619892683","article-title":"Diagnostic values of inflammatory and angiogenic factors for acute joint bleeding in patients with severe hemophilia A","volume":"26","author":"Xu H","year":"2020","journal-title":"Clin Appl Thromb Hemost"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2013.06.002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.03962.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200105000-00009"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-05-284653"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.bor.0000155361.83990.5b"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar756"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.12.4001"},{"key":"e_1_2_10_10_1","first-page":"1623","article-title":"Up\u2010regulation of endoglin on vascular endothelial cells in human solid tumors: implications for diagnosis and therapy","volume":"1","author":"Burrows FJ","year":"1995","journal-title":"Clin Cancer Res"},{"key":"e_1_2_10_11_1","first-page":"253","article-title":"Functional analysis of the TGFbeta receptor\/Smad pathway through gene ablation in mice","volume":"44","author":"Goumans MJ","year":"2000","journal-title":"Int J Dev Biol"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00562-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700867RR"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4274\/tjh.2016.0031"},{"key":"e_1_2_10_15_1","unstructured":"Daly Cad S. 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