{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:10:46Z","timestamp":1747185046596,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T00:00:00Z","timestamp":1635120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Studying the pathological changes of ligaments in patients with haemophilic arthritis (HA)\u00a0has important significance for guiding the release of ligaments during total knee arthroplasty (TKA) and exploring interventions to prevent ligament lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study was conducted to show the pathological changes and investigate the lysine oxidase (LOX) and matrix metalloproteinase (MMP)\u20101, \u20102, and \u20103 levels in the ligaments of patients with HA compared with those of patients with osteoarthritis (OA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ligaments obtained during the TKA were stained with Masson trichrome, Verhoeff\u2010Van Gieson and haematoxylin and eosin to show\u00a0the\u00a0basic pathological changes. Collagen I, elastin, LOXs and MMP\u20101, \u20102, and \u20103 expression levels were detected via western blot. LOX and MMP\u20101, \u20102, and \u20103 mRNA expression levels were analysed via quantitative real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with OA ligaments, HA ligaments\u00a0were constructed\u00a0more loosely with wider gaps, more breaks, haemocytodeposition and local hypertrophy\u00a0among\u00a0the fibres. LOXs and MMP mRNA expression levels were upregulated in the HA tissues, which was consistent with the western blot results. Collagen I and elastin levels were also higher in patients with HA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The metabolism of the ligaments\u00a0in patients with HA is more complex than in those with OA, and the ligaments of patients with HA have stronger healing and destruction processes. This pathology is related to iron overload and imbalanced inflammatory factors due to repeated intra\u2010articular bleeding.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.14443","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T04:27:05Z","timestamp":1635222425000},"page":"145-150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Pathological changes and expression of lysine oxidases and matrix metalloproteinases \u20101, \u20102, and \u20103 in ligaments of patients with haemophilic arthritis"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9223-8782","authenticated-orcid":false,"given":"Liujie","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery The Second Hospital of Anhui Medical University  Hefei Anhui 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