{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:29:44Z","timestamp":1783002584823,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Elevated markers of endothelial dysfunction and inflammation indicate worse endothelial function in the aging haemophilia population. MicroRNAs (miRNAs) regulate gene expression post\u2010transcriptionally. Several miRNAs have been shown to be involved in the process of endothelial dysfunction and atherosclerosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study was to determine the underlying molecular pathways of endothelial dysfunction and inflammation in haemophilia patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 25 patients with severe or moderate haemophilia A (20 patients) or B (5 patients), 14 controls and 18 patients with coronary artery disease (CAD) after myocardial infarction were included in this study. Expression of miRNA\u2010126, \u2010155, \u2010222, \u20101, \u2010let7a, \u201021 and \u2010197 were analysed using a real time polymerase chain reaction. Network\u2010based visualisation and analysis of the miRNA\u2010target interactions were performed using the MicroRNA ENrichment TURned NETwork (MIENTURNET).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of miRNA\u2010126 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and miRNA\u2010let7a (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) were significantly higher in CAD patients compared to haemophilia patients and controls. MiRNA\u201021 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) was significantly elevated in CAD patients compared to controls. MiRNA\u2010155 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), miRNA\u20101 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and miRNA\u2010197 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) were significantly higher expressed in CAD and haemophilia patients compared to controls and showed a strong correlation with increased levels of interleukin\u20106 (IL\u20106) and soluble intercellular adhesion molecule\u20101 (sICAM\u20101). The network analysis revealed interactions in the cytokine signalling, focal adhesion and VEGFA\u2010VEGFR2 pathway (Vascular endothelial growth factor, \u2010receptor).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study characterises miRNA expression in haemophilia patients in comparison to CAD patients and healthy controls. The results imply comparable biological processes in CAD and haemophilia patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.14658","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T18:39:22Z","timestamp":1663353562000},"page":"61-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Endothelial dysfunction and atherosclerosis related miRNA\u2010expression in patients with haemophilia"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6755-0539","authenticated-orcid":false,"given":"Stephanie","family":"Noone","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology Intensive Care Medicine and Pain Therapy University Hospital Frankfurt Goethe University  Frankfurt Germany"},{"name":"Division of Haemostaseology Department of Internal Medicine II University Hospital Frankfurt Goethe University  Frankfurt 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