{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:11:54Z","timestamp":1747185114361,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,24]],"date-time":"2024-03-24T00:00:00Z","timestamp":1711238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21K07804","22K16330"],"award-info":[{"award-number":["21K07804","22K16330"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Emicizumab (Emi) is used as haemostatic prophylaxis for patients with haemophilia A (PwHA). Disseminated intravascular coagulation (DIC) is a condition characterized by persistent systemic activation of coagulation, but there is yet no information on coagulation and fibrinolysis potentials in Emi\u2010treated PwHA with DIC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To examine the effect of Emi on coagulation and fibrinolysis potentials in HA\u2010model DIC plasmas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma from a patient with sepsis\u2010DIC (seven patients) was treated with anti\u2010factor (F)VIII monoclonal antibody (HA\u2010model DIC plasma) and incubated with Emi (50\u00a0\u00b5g\/mL). The plasma was then assessed using clot\u2010fibrinolysis waveform analysis (CFWA). Coagulation and fibrinolysis parameters were expressed as ratios relative to normal plasma (|min1|\u2010ratio and |FL\u2010min1|\u2010ratio, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and results<\/jats:title><jats:p>In case 1, coagulant potential was slightly high and fibrinolytic potential was extremely low, presenting a coagulant\u2010dominant state (|min1|\u2010ratio\/|FL\u2010min1|\u2010ratio: 1.1\/.38). In cases 2\u20135, fibrinolytic potential was not suppressed, but there were marked hypercoagulant potentials, indicating relative coagulant\u2010dominant states. In case 6, coagulant and fibrinolytic potentials were increased but well balanced (|min1|\u2010ratio\/|FL\u2010min1|\u2010ratio: 1.38\/1.28). In case 7, both potentials were severely deteriorated in not only CFWA but also the thrombin\/plasmin generation assay. The addition of Emi into the HA\u2010model DIC plasmas increased |min1|\u2010ratio values in all cases, but the coagulant potentials did not exceed the initial ones (DIC plasma before treatment with anti\u2010FVIII antibody).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The presence of Emi in the HA\u2010model DIC plasma improved coagulation potentials, but did not increase coagulation potentials beyond those of DIC plasma in non\u2010HA states.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hae.14997","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T04:29:45Z","timestamp":1711340985000},"page":"836-844","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The effects of emicizumab on in vitro coagulation and fibrinolysis parameters in patients with disseminated intravascular coagulation with and without addition of anti\u2010FVIII 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Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2415-2194","authenticated-orcid":false,"given":"Keiji","family":"Nogami","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nara Medical University  Kashihara Nara 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