{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:38:23Z","timestamp":1792654703556,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Von Willebrand disease (vWD) is a common bleeding disorder with different subtypes. Laboratory diagnosis is challenging, involving several expensive and complex assays. The von Willebrand factor (vWF) collagen binding assay (VWF:CB) has been described to improve the diagnosis of vWD, but there is a lack of consensus and its implementation into guidelines and diagnostic algorithms is incomplete.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A cohort of 88 patients with inherited vWD and 10 patients investigated for vWD due to a bleeding phenotype were recruited and underwent analysis of vWF multimers, vWF antigen (VWF:Ag) and VWF:CB. The total bleeding score (BS) was calculated by using the bleeding assessment tool recommended by the International Society on Thrombosis and Haemostasis. An optimal VWF:CB\/VWF:Ag ratio cutoff for differentiating between patients with all multimer sizes and those lacking high molecular weight multimers (HMWM) was established, and the findings were validated in a separate retrospective clinical data cohort. The association between VWF:CB\/VWF:Ag ratio and BS was also assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>VWF:CB\/VWF:Ag ratio was a very good discriminator of HMWM presence, yielding a sensitivity of 1.0 and a specificity of 0.79 at the optimal cutoff in the validation dataset.<\/jats:p>\n                    <jats:p>\n                      VWF:CB\/VWF:Ag ratio was a significant predictor of BS (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      = 0.39).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>VWF:CB\/VWF:Ag ratio is a significant predictor of BS and multimer analysis can be safely omitted in patients with vWD and a VWF:CB\/VWF:Ag ratio above 0.6, confirming the VWF:CB assay as an important contributor to vWD diagnosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hae.70025","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T05:30:10Z","timestamp":1741757410000},"page":"450-457","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ratio of Von Willebrand Collagen Binding Assay and Von Willebrand Antigen Can Predict Multimer Size in Von Willebrand Disease"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8367-4217","authenticated-orcid":false,"given":"Adrian","family":"Kimiaei","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Molecular Medicine and Surgery Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iva","family":"Pruner","sequence":"additional","affiliation":[{"name":"Center for Clinical and Translational Research the Abigail Wexner Research Institute at Nationwide Children's Hospital  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Farm","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margareta","family":"Holmstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Hematology Coagulation Unit Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Health Medicine and Caring Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natali","family":"Karandyszowska","sequence":"additional","affiliation":[{"name":"Department of Hematology Coagulation Unit Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Medicine Solna Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Bruzelius","sequence":"additional","affiliation":[{"name":"Department of Hematology Coagulation Unit Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Medicine Solna Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovan","family":"Antovic","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry Karolinska University Hospital  Stockholm Sweden"},{"name":"Department of Molecular Medicine and Surgery Karolinska Institute  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"\u00c5gren","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery Karolinska Institute  Stockholm Sweden"},{"name":"Department of Hematology Coagulation Unit Karolinska University Hospital  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1601561"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.2.454.454"},{"key":"e_1_2_11_4_1","first-page":"440","volume-title":"Seminars in Thrombosis and Hemostasis","author":"Favaloro E. 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