{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:40:55Z","timestamp":1773009655354,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"published-print":{"date-parts":[[2025,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n The Bethesda assay (BA) and the Nijmegen\u2010Bethesda assay (NBA) are commonly used to measure inhibitors in patients with haemophilia A (HA). The laboratory results of inhibitor assays have been demonstrated to be highly variable.<\/jats:p>\n <\/jats:sec>\n \n Aims<\/jats:title>\n To understand the current testing methods used in different laboratories in China and promote improvements in laboratory detection.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n An external quality assurance (EQA) survey of FVIII inhibitor testing was conducted by the Chinese Alliance for Blood Disease and Tianjin Blood Disease Medical Quality Control Centre to evaluate the testing methods used.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Seventy\u2010eight questionnaires and matched EQA responses were received. A total of 94.9% of participating centres reported the use of BA for the FVIII inhibitor assay. Five EQA samples revealed titres and coefficients of variation (CVs) of 0.7\u00a0BU\/mL (0\u20131.9\u00a0BU\/mL; CV\u00a0=\u00a063.9%), 2.0\u00a0BU\/mL (0.6\u20134.8\u00a0BU\/mL; CV\u00a0=\u00a040.7%), 5.6 (2.3\u201311.6 BU\/mL, CV\u00a0=\u00a033.4%), 14.6 (4.8\u201327.2\u00a0BU\/mL, CV = 32.8%) and 37.2 (11.5\u201380.0\u00a0BU\/mL, CV\u00a0=\u00a035.2%), respectively. About 37% laboratories failed to detect low\u2010level inhibitors of approximately 1\u00a0BU\/mL. The overall results of Dade Actin were lower than those of other APTT reagents.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n There is a wide variety in the stages of inhibitor testing, and further local standardization is required to improve interlaboratory comparison of factor VIII inhibitor assay.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/hae.70084","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T03:55:23Z","timestamp":1751946923000},"page":"1086-1091","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nationwide Survey of Laboratory Practices for Factor VIII Inhibitor Detection in China"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0098-1213","authenticated-orcid":false,"given":"Yuhua","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Centre for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Hematologic Pathology Centre Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyao","family":"Kong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Centre for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Hematologic Pathology Centre Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyuan","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Centre for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Hematologic Pathology Centre Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunjun","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Centre for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Hematologic Pathology Centre Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Centre for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Hematologic Pathology Centre Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin 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Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Tianjin Key Laboratory of Gene Therapy For Blood Diseases, CAMS Key Laboratory of Gene Therapy For Blood Diseases Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5947-3924","authenticated-orcid":false,"given":"Feng","family":"Xue","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Centre for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin China"},{"name":"Tianjin Institutes of Health Science Tianjin China"},{"name":"Tianjin Key Laboratory of Gene Therapy For Blood Diseases, CAMS Key Laboratory of Gene Therapy For Blood Diseases Tianjin 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