{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T07:53:57Z","timestamp":1781510037572,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Haemophilia"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Inter\u2010individual variability in factor VIII (FVIII) pharmacokinetics (PK) is well recognized in people with haemophilia A (PWH\u2010A) and may compromise the effectiveness of prophylaxis. This variability is partially explained by clinical and biological determinants, including age, anthropometric parameters and von Willebrand factor antigen (VWF:Ag) levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To identify the determinants of FVIII\u2010PK in Tunisian PWH\u2010A and assess their implications for PK\u2010guided prophylaxis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A prospective PK study was conducted in 25 people with severe or moderate haemophilia A enrolled between December 2022 and December 2024. Following a 72\u2010hour wash\u2010out, PWH\u2010A received standard half\u2010life FVIII concentrates. FVIII activity was measured using one\u2010stage assay (OSA) and chromogenic substrate assay (CSA) at baseline, 30\u00a0min, 5\u00a0h \u00b11\u00a0h, 24\u00a0h and 48\u00a0h post\u2010infusion. PK parameters\u2014half\u2010life, clearance, in vivo recovery and area under curve (AUC)\u2010 were calculated and correlated with age, anthropometric parameters, ABO blood group and VWF:Ag levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      FVIII activity measured by OSA and CSA showed a strong correlation (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.0001). Higher VWF:Ag levels were associated with longer FVIII half\u2010life (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.021) and lower clearance (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.027). Body mass index (BMI) correlated positively with IVR (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.007) while younger age was associated with higher clearance (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.037). No significant differences in PK parameters were observed between plasma\u2010derived and recombinant FVIII concentrates, except for higher AUC with plasma\u2010derived concentrates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>FVIII PK in Tunisian PWH\u2010A shows substantial inter\u2010individual variability. VWF:Ag levels, age and BMI were associated with PK parameters, supporting the implementation of individualized PK\u2010guided prophylaxis in Tunisia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hae.70335","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T07:25:23Z","timestamp":1781508323000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Beyond Body Weight: Biological and Clinical Determinants of Factor VIII Pharmacokinetics in Tunisian Patients"],"prefix":"10.1111","author":[{"given":"Sameh","family":"Neji","sequence":"first","affiliation":[{"name":"Haemophilia Treatment Centre Aziza Othmana Hospital  Tunis Tunisia"},{"name":"Department of Hematology Aziza Othmana Hospital  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ons","family":"Ghali","sequence":"additional","affiliation":[{"name":"Haemophilia Treatment Centre Aziza Othmana Hospital  Tunis Tunisia"},{"name":"Department of Hematology Aziza Othmana Hospital  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha","family":"Said","sequence":"additional","affiliation":[{"name":"Haemophilia Treatment Centre Aziza Othmana Hospital  Tunis Tunisia"},{"name":"Department of Hematology Aziza Othmana Hospital  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wijdene El","family":"Borji","sequence":"additional","affiliation":[{"name":"Department of Hematology Aziza Othmana Hospital  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amel","family":"Chaabane","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Fattouma Bourguiba Hospital  Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emna","family":"Gouider","sequence":"additional","affiliation":[{"name":"Haemophilia Treatment Centre Aziza Othmana Hospital  Tunis Tunisia"},{"name":"Department of Hematology Aziza Othmana Hospital  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010021\u201000278\u2010x"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/hae.14046"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006745"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102516.2006.01401.x"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2009.03703.x"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2018.01.001"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228\u2010009\u20100676\u2010x"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538\u20107836.2010.03757.x"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019000190"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.15183"},{"issue":"3","key":"e_1_2_12_12_1","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1055\/s-0038-1646423","article-title":"The Design and Analysis of Half\u2010life and Recovery Studies for Factor VIII and Factor IX. 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