{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:12:34Z","timestamp":1747185154261,"version":"3.40.5"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hemodialysis International"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>We evaluated the effects of pre\u2010analytical care on total carbon dioxide (tCO<jats:sub>2<\/jats:sub>) in hemodialysis patients, as calculated by blood gas analysis (ctCO<jats:sub>2<\/jats:sub>) or measured by an enzymatic assay (mtCO<jats:sub>2<\/jats:sub>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples were collected via vascular access before dialysis sessions. For blood gas analysis, eight aliquots were collected, refrigerated or non\u2010refrigerated, and analyzed at 0, 4, 8, and 24\u2009h after collection. A blood sample was then collected for the enzymatic method and distributed into 14 aliquots. Half of the aliquots were refrigerated. The samples analyzed at time point 0 were centrifuged immediately. The remaining aliquots of both the refrigerated and non\u2010refrigerated clusters were centrifuged before storage. Samples were analyzed at 4, 8, and 24\u2009h post\u2010collection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Findings<\/jats:title><jats:p>By blood gas analysis, no significant change was found in bicarbonate values over time, either in the non\u2010refrigerated or refrigerated samples. ctCO<jats:sub>2<\/jats:sub> values during the experiment showed a minor but statistically significant increase of questionable clinical relevance in both non\u2010refrigerated and refrigerated aliquots. In the enzymatic assay, the reduction in mtCO<jats:sub>2<\/jats:sub> levels during the experiment was negligible. The median absolute reductions at the end of the experiment were 1.77, 1.21, 1.04, and 1.12\u2009mmol\/L for the non\u2010centrifuged\/non\u2010refrigerated, centrifuged\/non\u2010refrigerated, non\u2010centrifuged\/refrigerated, and centrifuged\/refrigerated aliquots, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Our results suggest that measured or calculated tCO<jats:sub>2<\/jats:sub> levels of capped and cooled samples are adequate for analyzing the acid\u2013base status of hemodialysis patients, even when such determination is not performed immediately after collection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hdi.13069","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T04:34:50Z","timestamp":1676435690000},"page":"105-111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of pre\u2010analytical sample care and analysis methodology on measures of metabolic acidosis in hemodialysis patients"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1574-9914","authenticated-orcid":false,"given":"Camilla C.","family":"Correa","sequence":"first","affiliation":[{"name":"Universidade Federal Fluminense Medical Science Post\u2010Graduation Program, Department of Medicine  Niteroi Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana M. B.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Nephrology Division, Department of Medicine  Niteroi Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Analucia R.","family":"Xavier","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Clinical Biochemistry, Department of Pathology  Niteroi Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salim","family":"Kanaan","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Clinical Biochemistry, Department of Pathology  Niteroi Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jocemir R.","family":"Lugon","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Nephrology Division, Department of Medicine, Medical School  Niteroi Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/sdi.12373"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V1071606"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(04)00936-9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2013.03.035"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.05780612"},{"key":"e_1_2_7_7_1","unstructured":"ChrisHiggins.Parameters that reflect the carbon dioxide content of blood. acutecaretesting.org [internet] 2008;1\u20139. [cited 2022 Sep 6] Available from:https:\/\/acutecaretesting.org\/en\/articles\/parameters-that-reflect-the-carbon-dioxide-content-of-blood."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.107441"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(00)70041-2"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(96)90251-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/7.3.336"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/diseases4040036"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000070205"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.00010505"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.317.7167.1213"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2008.005"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2020.40.6.448"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.5812\/ircmj.13577"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12871-018-0655-9"}],"container-title":["Hemodialysis International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hdi.13069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/hdi.13069","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hdi.13069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T14:56:47Z","timestamp":1692716207000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hdi.13069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,14]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1111\/hdi.13069"],"URL":"https:\/\/doi.org\/10.1111\/hdi.13069","archive":["Portico"],"relation":{},"ISSN":["1492-7535","1542-4758"],"issn-type":[{"type":"print","value":"1492-7535"},{"type":"electronic","value":"1542-4758"}],"subject":[],"published":{"date-parts":[[2023,2,14]]},"assertion":[{"value":"2022-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}