{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:32:22Z","timestamp":1786635142584,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["headachejournal.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Headache"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the impact of neuropathic lesion on cortical synchronization in processing spontaneous pain\u2010like behavior.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In vivo optical monitoring of neuronal activity may provide insightful mechanisms underlying the complexity of spontaneous pain\u2010like behavior in freely moving animals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We examined the synchronized pattern of the pyramidical neurons in anterior cingulate cortex during spontaneous grooming behavior using optical monitoring of Ca\n                      <jats:sup>2+<\/jats:sup>\n                      activity. A chronic constriction injury of infraorbital nerve model was performed to induce trigeminal neuropathic pain. We then analyzed the synchronized patterns of cortical population and computed Shannon entropy values to assess the uncertainty of neural coding during spontaneous pain\u2010like behavior.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following nerve injury, mice exhibited significantly prolonged isolated grooming behavior compared to the control group. Our data indicate that, while neuropathic pain enhanced synchronized activity of cortical network during spontaneous grooming behavior, craniofacial nociception reduced the uncertainty of neural firing. Interestingly, this transition to a synchronized state of cortical ensembles in neuropathic pain conditions was significantly disrupted by spontaneous grooming behavior.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings provided a method of monitoring the synchronized activity of cortical ensemble in freely moving animals. Synchronization index may be used to decode spontaneous neuropathic pain\u2010like behavior.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/head.70084","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T06:36:51Z","timestamp":1773643011000},"page":"1108-1116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cortical synchronization of spontaneous trigeminal neuropathic pain monitored with in\u00a0vivo optical imaging of calcium activity in freely moving mice"],"prefix":"10.1111","volume":"66","author":[{"given":"Qianxi","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pain, The Third Xiangya Hospital and Institute of Pain Medicine Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pain, The Third Xiangya 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