{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:58:39Z","timestamp":1762459119469},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T00:00:00Z","timestamp":1383177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The envisaged roles and partly understood functional properties of <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>elicobacter pylori<\/jats:italic> protein HP0986 are significant in the context of proinflammatory and or proapoptotic activities, the two important facilitators of pathogen survival and persistence. In addition, sequence analysis of this gene predicts a restriction endonuclease function which remained unknown thus far. To evaluate the role of HP0986 in gastric inflammation, we studied its expression profile using a large number of clinical isolates but a limited number of biopsies and patient sera. Also, we studied antigenic role of HP0986 in altering cytokine responses of human gastric epithelial (<jats:styled-content style=\"fixed-case\">AGS<\/jats:styled-content>) cells including its interaction with and localization within the <jats:styled-content style=\"fixed-case\">AGS<\/jats:styled-content> cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>For <jats:italic>in vitro<\/jats:italic> expression study of HP0986, 110 <jats:italic>H.\u00a0pylori<\/jats:italic> clinical isolates were cultured from patients with functional dyspepsia. For expression analysis by q<jats:styled-content style=\"fixed-case\">RT PCR<\/jats:styled-content> of HP0986, 10 gastric biopsy specimens were studied. HP0986 was also used to detect antibodies in patient sera. <jats:styled-content style=\"fixed-case\">AGS<\/jats:styled-content> cells were incubated with recombinant HP0986 to determine cytokine response and NF\u2010\u03baB activation. Transient transfection with HP0986 cloned in pEGFPN1 was used to study its subcellular localization or homing in <jats:styled-content style=\"fixed-case\">AGS<\/jats:styled-content> cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 110 cultured <jats:italic>H.\u00a0pylori<\/jats:italic> strains, 34 (31%) were positive for HP0986 and this observation was correlated with <jats:italic>in vitro<\/jats:italic> expression profiles. HP0986 mRNA was detected in 7 of the 10 biopsy specimens. Further, HP0986 induced IL\u20108 secretion in gastric epithelial cells in a dose and time\u2010dependent manner <jats:italic>via<\/jats:italic> NF\u2010\u03baB pathway. Serum antibodies against HP0986 were positively associated with <jats:italic>H.\u00a0pylori<\/jats:italic> positive patients. Transient transfection of <jats:styled-content style=\"fixed-case\">AGS<\/jats:styled-content> cells revealed both cytoplasmic and nuclear localization of HP0986.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HP0986 was moderately prevalent in clinical isolates and its expression profile in cultures and gastric biopsies points to its being naturally expressed. Collective observations including the induction of IL\u20108 <jats:italic>via <\/jats:italic><jats:styled-content style=\"fixed-case\">TNFR<\/jats:styled-content>1 and NF\u2010\u03baB, subcellular localization, and seropositivity data point to a significant role of HP0986 in gastroduodenal inflammation. We propose to name the HP0986 gene\/protein as \u2018TNFR1 interacting endonuclease A (TieA or <jats:italic>tie<\/jats:italic>A)\u2019.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12100","type":"journal-article","created":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T11:05:38Z","timestamp":1383217538000},"page":"26-36","source":"Crossref","is-referenced-by-count":18,"title":["<i>Helicobacter pylori<\/i> Antigen HP0986 (TieA) Interacts with Cultured Gastric Epithelial Cells and Induces IL8 Secretion <i>via<\/i> NF\u2010\u03baB Mediated Pathway"],"prefix":"10.1111","volume":"19","author":[{"given":"Savita","family":"Devi","sequence":"first","affiliation":[{"name":"Pathogen Biology Laboratory Department of Biotechnology and Bioinformatics University of Hyderabad Hyderabad 500046 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suhail A.","family":"Ansari","sequence":"additional","affiliation":[{"name":"Pathogen Biology Laboratory Department of Biotechnology and Bioinformatics University of Hyderabad Hyderabad 500046 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamuna","family":"Vadivelu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Faculty of Medicine University of Malaya Kuala Lumpur 50603 Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"M\u00e9graud","sequence":"additional","affiliation":[{"name":"INSERM \u2010 U853 Laboratoire de Bacteriologie \u2010 C.H.U. 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