{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:32:38Z","timestamp":1790400758533,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T00:00:00Z","timestamp":1391558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Outer inflammatory protein A (OipA) has an important role in\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\n                        elicobacter pylori\n                      <\/jats:italic>\n                      pathogenesis. In this study, we purified the outer membrane protein and evaluated the effects of this protein on maturation and cytokine production by dendritic cells (\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      s).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>oipA<\/jats:italic>\n                      gene was inserted into\n                      <jats:styled-content style=\"fixed-case\">pET<\/jats:styled-content>\n                      28a, and this construct was transformed into\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>\n                        scherichia coli\n                      <\/jats:italic>\n                      BL21 (DE3). Purification of the recombinant protein was performed by Ni\u2010NTA affinity chromatography. Immature DCs were purified from spleen of C57BL\/6 mice with more than 90% purity and were treated with several concentrations of OipA (1\u201320\u00a0\u03bcg\/mL) overnight. Expression of maturation markers (CD86, CD40, and MHC\u2010II) on the surface of DCs and production of IL\u201010 and IL\u201012 were assessed by flow cytometry and ELISA, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The expression of\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      maturation markers\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      40,\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      86, and\n                      <jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\n                      was downregulated on the surface of OipA\u2010treated\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      s at concentrations of 10 and 20\u00a0\u03bcg\/mL compared with negative control. Production of\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201010 decreases with increasing OipA concentration at a concentration of 5\u00a0\u03bcg\/mL, but we detected no change in\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201012 production.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Inability to eliminate\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\n                        .\u00a0pylori\n                      <\/jats:italic>\n                      from stomach is partly due to the evasion of the bacteria from the immune response.\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      s are central mediators between innate and adaptive immunity, and\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      cytokines direct the types of adaptive immune response. This study indicated that OipA of\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\n                        .\u00a0pylori\n                      <\/jats:italic>\n                      is a\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      maturation suppression factor. Previous studies have shown that\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\n                        .\u00a0pylori\n                      <\/jats:italic>\n                      manage tolerogenic programming in\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      s leading to long\u2010time gastric colonization. In conclusion,\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\n                        .\u00a0pylori\n                      <\/jats:italic>\n                      OipA helps the establishment of chronic infection with reduction in\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201010 and suppression of\n                      <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\n                      maturation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hel.12107","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T01:20:30Z","timestamp":1391563230000},"page":"136-143","source":"Crossref","is-referenced-by-count":32,"title":["In Vitro Suppression of Dendritic Cells by\n                    <i>Helicobacter pylori<\/i>\n                    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