{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T02:43:01Z","timestamp":1781664181599,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T00:00:00Z","timestamp":1400457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council Taiwan","doi-asserted-by":"publisher","award":["NSC 100-2113-M-001-022-MY3"],"award-info":[{"award-number":["NSC 100-2113-M-001-022-MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The <jats:italic>lon<\/jats:italic> gene of <jats:italic>Helicobacter pylori<\/jats:italic> strains is constitutively expressed during growth. However, virtually nothing is understood concerning the role of Lon in <jats:italic>H.\u00a0pylori<\/jats:italic>. This study examined the function and physiological role of Lon in <jats:italic>H.\u00a0pylori<\/jats:italic> (HpLon) using a trapping approach to identify putative Lon binding partners in the bacterium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Protease\u2010deficient Lon was expressed and served as the bait in trapping approach to capture the interacting partners in <jats:italic>H.\u00a0pylori<\/jats:italic>. The antibiotic susceptibility of wild\u2010type and <jats:italic>lon<\/jats:italic> derivative mutants was determined by the E test trips and the disc diffusion assay. The effect of HpLon on RdxA activity was detected the change in <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidation and metronidazole reduction by spectrophotometer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Lon in <jats:italic>Helicobacter pylori<\/jats:italic> (HpLon) interacting partners are mostly associated with metronidazole activation. <jats:italic>lon<\/jats:italic> mutant presents more susceptible to metronidazole than that of the wild type, and this phenotype is recovered by complementation of the wild\u2010type Lon. We found that the ATPases associated with a variety of cellular activities (<jats:styled-content style=\"fixed-case\">AAA<\/jats:styled-content><jats:sup>+<\/jats:sup>) module of HpLon causes a decrease in both <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase and Mtz reductase activity in RdxA, a major Mtz\u2010activating enzyme in <jats:italic>H.\u00a0pylori<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Metronidazole resistance of <jats:italic>H.\u00a0pylori<\/jats:italic> causes the serious medical problem worldwide. In this study, HpLon is involved in metronidazole susceptibility among <jats:italic>H.\u00a0pylori<\/jats:italic> strains. We provide the evidence that HpLon alters RdxA activity in vitro. The decrease in metronidazole activation caused by HpLon is possibly prior to accumulate mutation in <jats:italic>rdxA<\/jats:italic> gene before the metronidazole\u2010resistant strains to be occurred.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12140","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T06:30:51Z","timestamp":1400481051000},"page":"356-366","source":"Crossref","is-referenced-by-count":7,"title":["Lon Protease Affects the <scp>R<\/scp>dx<scp>A<\/scp> Nitroreductase Activity and Metronidazole Susceptibility in <i>Helicobacter pylori<\/i>"],"prefix":"10.1111","volume":"19","author":[{"given":"I\u2010Fan","family":"Tu","sequence":"first","affiliation":[{"name":"Institute of Bioinformatics and Structural Biology National Tsing Hua University Hsinchu 300 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahn\u2010Haur","family":"Liao","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry 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