{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:19:38Z","timestamp":1791335978158,"version":"4.3.1"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T00:00:00Z","timestamp":1406073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003715","name":"Korea Research Institute of Bioscience and Biotechnology","doi-asserted-by":"publisher","award":["KGM1211012"],"award-info":[{"award-number":["KGM1211012"]}],"id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korean Health Technology R&D Project, Ministry of Health & Welfare","award":["A120176"],"award-info":[{"award-number":["A120176"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>.\u00a0pylori<\/jats:italic>) infection plays an important role in the early stage of cancer development. However, various bacteria that promote the synthesis of reactive oxygen and nitrogen species may be involved in the later stages. We aimed to determine the microbial composition of gastric mucosa from the patients with chronic gastritis, intestinal metaplasia, and gastric cancer using 454 <jats:styled-content style=\"fixed-case\">GS FLX<\/jats:styled-content> Titanium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gastric mucosal biopsy samples were collected from 31 patients during endoscopy. After the extraction of genomic DNA, variable region V5 of the 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene was amplified. PCR products were sequenced using 454 high\u2010throughput sequencer. The composition, diversity, and richness of microbial communities were compared between three groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The composition of <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>.\u00a0pylori<\/jats:italic>\u2010containing <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>psilonproteobacteria<\/jats:italic> class appeared to be the most prevalent, but the relative increase in the <jats:italic>Bacilli<\/jats:italic> class in the gastric cancer group was noticed, resulting in a significant difference compared with the chronic gastritis group. By analyzing the <jats:italic>Helicobacter<\/jats:italic>\u2010dominant group at a family level, the relative abundance of <jats:italic>Helicobacteraceae<\/jats:italic> family was significantly lower in the gastric cancer group compared with chronic gastritis and intestinal metaplasia groups, while the relative abundance of <jats:italic>Streptococcaceae<\/jats:italic> family significantly increased. In a <jats:styled-content style=\"fixed-case\">UPGMA<\/jats:styled-content> clustering of <jats:italic>Helicobacter<\/jats:italic>\u2010dominant group based on UniFrac distance, the chronic gastritis group and gastric cancer group were clearly separated, while the intestinal metaplasia group was distributed in between the two groups. The evenness and diversity of gastric microbiota in the gastric cancer group was increased compared with other groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In <jats:italic>Helicobacter<\/jats:italic> predominant patients, the microbial compositions of gastric mucosa from gastric cancer patients are significantly different to chronic gastritis and intestinal metaplasia patients. These alterations of gastric microbial composition may play an important, as\u2010yet\u2010undetermined role in gastric carcinogenesis of <jats:italic>Helicobacter<\/jats:italic> predominant patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12145","type":"journal-article","created":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T12:14:42Z","timestamp":1406117682000},"page":"407-416","source":"Crossref","is-referenced-by-count":275,"title":["Differences in Gastric Mucosal Microbiota Profiling in Patients with Chronic Gastritis, Intestinal Metaplasia, and Gastric Cancer Using Pyrosequencing Methods"],"prefix":"10.1111","volume":"19","author":[{"given":"Chang Soo","family":"Eun","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Hanyang University Guri Hospital Gyeonggi\u2010Do Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byung Kwon","family":"Kim","sequence":"additional","affiliation":[{"name":"Bionano Health Guard Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Soo","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Hanyang University Guri Hospital Gyeonggi\u2010Do Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seon Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Medical Genomics Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Mo","family":"Kim","sequence":"additional","affiliation":[{"name":"Biological Resource Center Korea Research Institute of Bioscience and Biotechnology Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo Youl","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine Hanyang University College of Medicine Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu Sang","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pathology Chungnam National University College of Medicine Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"Medical Genomics Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihyun F.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Division of Life Sciences Yonsei University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,23]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"6735","article-title":"Human gastric carcinogenesis: a multistep and multifactorial process \u2013 first American Cancer Society 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