{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T22:03:23Z","timestamp":1785017003103,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T00:00:00Z","timestamp":1421020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Research Fund of Ghent University","award":["BOF08\/GOA\/004 and 01W00310"],"award-info":[{"award-number":["BOF08\/GOA\/004 and 01W00310"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Helicobacter<\/jats:italic> (<jats:italic>H.) suis<\/jats:italic> causes gastritis and decreased weight gain in pigs. It is also the most prevalent non\u2010<jats:italic>Helicobacter pylori Helicobacter<\/jats:italic> species in humans with gastric disease. <jats:italic>H.\u00a0suis<\/jats:italic> is extremely fastidious, and so far, biphasic culture conditions were essential for isolation and culture, making it impossible to obtain single colonies. Hence, cultures obtained from an individual animal may contain multiple <jats:italic>H.\u00a0suis<\/jats:italic> strains, which is undesirable for experiments aiming for instance at investigating <jats:italic>H.\u00a0suis<\/jats:italic> strain differences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Pure cultures of <jats:italic>H.\u00a0suis<\/jats:italic> were established by growing bacteria as colonies on 1% brucella agar plates, followed by purification and enrichment by biphasic subculture. Characteristics of these single colony\u2010derived strains were compared with those of their parent strains using multilocus sequence typing (<jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content>) and by studying bacterium\u2013host interactions using a gastric epithelial cell line and Mongolian gerbil model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The purification\/enrichment procedure required a nonstop culture of several weeks. For 4 of 17 <jats:italic>H.\u00a0suis<\/jats:italic> strains, <jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content> revealed differences between parental and single colony\u2010derived strains. For three of four single colony\u2010derived strains tested, the cell death\u2010inducing capacity was higher than for the parental strain. One single colony\u2010derived strain lost its capacity to colonize Mongolian gerbils. For the four other strains tested, colonization capacity and histopathologic changes were similar to what has been described when using strains with only a history of limited biphasic culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A method was developed to obtain single colony\u2010derived <jats:italic>H.\u00a0suis<\/jats:italic> strains, but this procedure may affect the bacterial genotype and phenotype.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12192","type":"journal-article","created":{"date-parts":[[2015,1,13]],"date-time":"2015-01-13T04:45:53Z","timestamp":1421124353000},"page":"206-216","source":"Crossref","is-referenced-by-count":12,"title":["Purification of <i><scp>H<\/scp>elicobacter suis<\/i> Strains From Biphasic Cultures by Single Colony Isolation: Influence on Strain Characteristics"],"prefix":"10.1111","volume":"20","author":[{"given":"Jungang","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Pathology, 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