{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:47:07Z","timestamp":1754398027034},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T00:00:00Z","timestamp":1425340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Federal Fund of the National Institute of Pediatrics.","award":["Project No.083-10"],"award-info":[{"award-number":["Project No.083-10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The genes <jats:italic>jhp0940<\/jats:italic>,<jats:italic> jhp0945<\/jats:italic>,<jats:italic> jhp0947<\/jats:italic>, and <jats:italic>jhp0949<\/jats:italic> belong to the plasticity region of the <jats:italic>Helicobacter pylori<\/jats:italic> genome. Due to their prevalence in isolates from patients with gastritis, duodenal ulcer, and gastric cancer, they have been proposed as markers of gastroduodenal diseases. These genes are associated with pro\u2010inflammatory cytokine induction through the <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB activation pathway. Nevertheless, the status of these genes is unknown in <jats:italic>H.\u00a0pylori<\/jats:italic> isolates from children. The <jats:italic>aim<\/jats:italic> of the present work was to determine the frequency of the <jats:italic>jhp0940<\/jats:italic>\u2010<jats:italic>jhp0945<\/jats:italic>\u2010<jats:italic>jhp0947<\/jats:italic>\u2010<jats:italic>jhp0949<\/jats:italic> genes in <jats:italic>H.\u00a0pylori<\/jats:italic> isolates from children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We identified the <jats:italic>jhp0940<\/jats:italic>,<jats:italic> jhp0945<\/jats:italic>,<jats:italic> jhp0947<\/jats:italic>, and <jats:italic>jhp0949<\/jats:italic> genes and the relationship of each with the virulence factors <jats:italic>cag<\/jats:italic>A, <jats:italic>cag<\/jats:italic><jats:styled-content style=\"fixed-case\">PAI<\/jats:styled-content>, and <jats:italic>dup<\/jats:italic>A by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in 49 isolates of <jats:italic>H.\u00a0pylori<\/jats:italic> from children. The results were corroborated using dot blots. In addition, we compared the prevalence of these genes with the prevalence in adults.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The prevalence of <jats:italic>jhp0940<\/jats:italic> (53.1%), <jats:italic>jhp0945<\/jats:italic> (44.9%), <jats:italic>jhp0947<\/jats:italic> (77.6%), and <jats:italic>jhp0949<\/jats:italic> (83.7%) was determined in the isolates from children, as was the prevalence of the virulence genes <jats:italic>cag<\/jats:italic>A (63.3%), <jats:italic>cag<\/jats:italic><jats:styled-content style=\"fixed-case\">PAI<\/jats:styled-content> (71.4%), and <jats:italic>dup<\/jats:italic>A (37.5%). No association was found between the four genes of the plasticity region and the virulence genes. The presence of the intact locus integrated by <jats:italic>jhp0940<\/jats:italic>\u2010<jats:italic>jhp0945<\/jats:italic>\u2010<jats:italic>jhp0947<\/jats:italic>\u2010<jats:italic>jhp0949<\/jats:italic> was very common among the isolates from children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The genes <jats:italic>jhp0940<\/jats:italic>,<jats:italic> jhp0947<\/jats:italic>, and <jats:italic>jhp0949<\/jats:italic> were present in more than 50% of the <jats:italic>H.\u00a0pylori<\/jats:italic> isolates, and the joint presence of <jats:italic>jhp0940\u2010jhp0945\u2010jhp0947\u2010jhp0949<\/jats:italic> was very frequent. The frequency of these genes in isolates from children could contribute to the virulence of <jats:italic>H.\u00a0pylori<\/jats:italic> and the evolution of the infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12194","type":"journal-article","created":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T01:36:54Z","timestamp":1425433014000},"page":"231-237","source":"Crossref","is-referenced-by-count":10,"title":["Plasticity Region Genes <i>jhp0940<\/i>,<i> jhp0945<\/i>,<i> jhp0947<\/i>, and <i>jhp0949<\/i> of <i><scp>H<\/scp>elicobacter pylori<\/i> in Isolates from <scp>M<\/scp>exican Children"],"prefix":"10.1111","volume":"20","author":[{"given":"Carolina","family":"Romo\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Experimental Bacteriology National Institute of Pediatrics SSA M\u00e9xico D.F. M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra","family":"Consuelo\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Gastroenterology &amp; Nutrition Department Children's Hospital of Mexico \u201cFederico G\u00f3mez\u201d SSA M\u00e9xico D.F. M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita","family":"Camorlinga\u2010Ponce","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Unit Instituto Mexicano del Seguro Social Mexico City Mexico, CMN Siglo XXI, IMSS, M\u00e9xico, D.F., M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norma","family":"Vel\u00e1zquez\u2010Guadarrama","sequence":"additional","affiliation":[{"name":"Infectology Laboratory Children's Hospital of M\u00e9xico \u201cFederico G\u00f3mez\u201d SSA M\u00e9xico D.F. 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