{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T06:38:33Z","timestamp":1778481513721,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T00:00:00Z","timestamp":1429833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005343","name":"Fundaci\u00f3n IMSS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005343","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2016,2]]},"abstract":"Abstract<\/jats:title>Background and aim<\/jats:title>Adherence to the gastric epithelium is one of the most important steps of H<\/jats:styled-content>elicobacter pylori<\/jats:italic> to remain and cause disease. The aim of this study was to analyze whether H.\u00a0pylori<\/jats:italic> isolates from patients with different gastroduodenal diseases present differences in the pattern of adherence to gastric epithelial cells (AGS<\/jats:styled-content>), in the ability to induce IL<\/jats:styled-content>\u20108, and in the presence of virulence genes.<\/jats:p><\/jats:sec>Methods<\/jats:title>We tested 75 H<\/jats:styled-content>.\u00a0pylori<\/jats:italic> strains isolated from nonatrophic gastritis, gastric cancer, and duodenal ulcer patients. The adhesion pattern and IL<\/jats:styled-content>\u20108 induction were determined in AGS<\/jats:styled-content> cells, and invasion of AGS<\/jats:styled-content> cells was studied using a gentamicin protection assay. The IL<\/jats:styled-content>\u20108 levels induced were determined by ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Helicobacter pylori<\/jats:italic> strains presented diffuse adherence (DA<\/jats:styled-content>) and localized (LA<\/jats:styled-content>) adherence patterns, similar to those described for enteropathogenic E<\/jats:styled-content>.\u00a0coli<\/jats:italic> (EPEC<\/jats:styled-content>), were observed in AGS<\/jats:styled-content> cells. A DA<\/jats:styled-content> pattern was observed in 57% and LA<\/jats:styled-content> in 43% of the strains, and DA<\/jats:styled-content> was more frequent in isolates from patients with gastric cancer (p<\/jats:italic>\u00a0=\u00a00.044). Strains with a LA<\/jats:styled-content> pattern induced higher levels of IL<\/jats:styled-content>\u20108 (p<\/jats:italic>\u00a0=\u00a00.042) in AGS<\/jats:styled-content> cells.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The adherence pattern was not associated with neither invasiveness nor with the presence of virulence genes. Our study shows that H<\/jats:styled-content>.\u00a0pylori<\/jats:italic> strains present adherence patterns to AGS<\/jats:styled-content> cells resembling those observed in EPEC<\/jats:styled-content> and that these patterns may be associated with disease and with activity on AGS<\/jats:styled-content> cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12230","type":"journal-article","created":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T06:44:02Z","timestamp":1429857842000},"page":"60-68","source":"Crossref","is-referenced-by-count":4,"title":["Patterns of Adherence of Helicobacter pylori<\/i> Clinical Isolates to Epithelial Cells, and its Association with Disease and with Virulence Factors"],"prefix":"10.1111","volume":"21","author":[{"given":"Flor Elizabeth","family":"V\u00e1zquez\u2010Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Infecciosas y Parasitarias UMAE, Hospital de Pediatria IMSS. M\u00e9xico, DF Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Torres","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Infecciosas y Parasitarias UMAE, Hospital de Pediatria IMSS. M\u00e9xico, DF Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lourdes","family":"Flores\u2010Luna","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica M\u00e9xico Morelos Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Giono Cerezo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Instituto Politecnico Nacional Escuela Nacional de Ciencias Biologicas Mexico, DF Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita","family":"Camorlinga\u2010Ponce","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Infecciosas y Parasitarias UMAE, Hospital de Pediatria IMSS. 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