{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T09:55:19Z","timestamp":1770717319457,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T00:00:00Z","timestamp":1441152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470831"],"award-info":[{"award-number":["81470831"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31070119"],"award-info":[{"award-number":["31070119"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The epitope vaccine is an attractive potential for prophylactic and therapeutic vaccination against <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H.\u00a0pylori<\/jats:italic>) infection. Lpp20 is one of major protective antigens which trigger immune response after <jats:italic>H.\u00a0pylori<\/jats:italic> invades host and has been considered as an excellent vaccine candidate for the control of <jats:italic>H.\u00a0pylori<\/jats:italic> infection. In our previous study, one B\u2010cell epitope and two <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T\u2010cell epitopes of Lpp20 were identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>In this study, an epitope vaccine composed of mucosal adjuvant cholera toxin B subunit (<jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>) and these three identified Lpp20 epitopes were constructed to investigate the efficacy of this epitope vaccine in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The epitope vaccine including <jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>, one B\u2010cell, and two <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T\u2010cell epitopes of Lpp20 was constructed and named <jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>\u2010Lpp20, which was then expressed in <jats:italic>Escherichia coli<\/jats:italic> and used for intraperitoneal immunization in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice. The immunogenicity, specificity, and ability to induce antibodies against Lpp20 and cytokine secretion were evaluated. After that, <jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>\u2010Lpp20 was intragastrically immunized to investigate the prophylactic and therapeutic efficacy in infected mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results indicated that the epitope vaccine <jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>\u2010Lpp20 possessed good immunogenicity and immunoreactivity and could elicit specific high level of antibodies against Lpp20 and the cytokine of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017. Additionally, <jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>\u2010Lpp20 significantly decreased <jats:italic>H.\u00a0pylori<\/jats:italic> colonization in <jats:italic>H.\u00a0pylori<\/jats:italic> challenging mice, and the protection was correlated with IgG, IgA, and <jats:styled-content style=\"fixed-case\">sIgA<\/jats:styled-content> antibody and Th1\u2010type cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study will be better for understanding the protective immunity of epitope vaccine, and <jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>\u2010Lpp20 may be an alternative strategy for combating <jats:italic>H.\u00a0pylori<\/jats:italic> invasion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12268","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T20:45:02Z","timestamp":1441226702000},"page":"234-248","source":"Crossref","is-referenced-by-count":34,"title":["Antibody Production and Th1\u2010biased Response Induced by an Epitope Vaccine Composed of Cholera Toxin B Unit and <i>Helicobacter pylori<\/i> Lpp20 Epitopes"],"prefix":"10.1111","volume":"21","author":[{"given":"Yan","family":"Li","sequence":"first","affiliation":[{"name":"School of Biotechnology Southern Medical University Guangzhou 510515 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongbiao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biotechnology Southern Medical University Guangzhou 510515 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbin","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Biotechnology Southern 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