{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:16:58Z","timestamp":1791317818026,"version":"4.3.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,2,23]],"date-time":"2016-02-23T00:00:00Z","timestamp":1456185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recent studies have shown that <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) activates signal transducer and activator of transcription 3 (STAT3) that plays an important role in gastric carcinogenesis. However, the molecular mechanism underlying <jats:italic>H. pylori<\/jats:italic>\u2010mediated STAT3 activation is still not fully understood. In this study, we investigated <jats:italic>H. pylori<\/jats:italic>\u2010induced activation of STAT3 signaling in AGS human gastric cancer cells and the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>AGS cells were cocultured with <jats:italic>H. pylori<\/jats:italic>, and STAT3 activation was assessed by Western blot analysis, electrophoretic mobility shift assay and immunocytochemistry. To demonstrate the involvement of reactive oxygen species (ROS) in <jats:italic>H. pylori<\/jats:italic>\u2010activated STAT3 signaling, the antioxidant <jats:italic>N<\/jats:italic>\u2010acetylcysteine was utilized. The expression and production of interleukin\u20106 (IL\u20106) were measured by reverse\u2010transcription polymerase chain reaction and enzyme\u2010linked immunosorbent assay (ELISA), respectively. The interaction between IL\u20106 and IL\u20106 receptor (IL\u20106R) was determined by the immunoprecipitation assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>H. pylori<\/jats:italic> activates STAT3 as evidenced by increases in phosphorylation on Tyr<jats:sup>705<\/jats:sup>, nuclear localization, DNA binding and transcriptional activity of this transcription factor. The nuclear translocation of STAT3 was also observed in <jats:italic>H. pylori<\/jats:italic>\u2010inoculated mouse stomach. In the subsequent study, we found that <jats:italic>H. pylori<\/jats:italic>\u2010induced STAT3 phosphorylation was dependent on IL\u20106. Notably, the increased IL\u20106 expression and the IL\u20106 and IL\u20106R binding were mediated by ROS produced as a consequence of <jats:italic>H. pylori<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>H. pylori<\/jats:italic>\u2010induced STAT3 activation is mediated, at least in part, through ROS\u2010induced upregulation of IL\u20106 expression. These findings provide a novel molecular mechanism responsible for <jats:italic>H. pylori<\/jats:italic>\u2010induced gastritis and gastric carcinogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12298","type":"journal-article","created":{"date-parts":[[2016,2,25]],"date-time":"2016-02-25T04:15:39Z","timestamp":1456373739000},"page":"405-416","source":"Crossref","is-referenced-by-count":69,"title":["<i>Helicobacter pylori<\/i> Activates IL\u20106\u2010STAT3 Signaling in Human Gastric Cancer Cells: Potential Roles for Reactive Oxygen Species"],"prefix":"10.1111","volume":"21","author":[{"given":"Juan\u2010Yu","family":"Piao","sequence":"first","affiliation":[{"name":"Research Institute of Pharmaceutical Sciences College of Pharmacy Seoul National University Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Geum","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Institute of Pharmaceutical Sciences College of Pharmacy Seoul 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