{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T10:46:56Z","timestamp":1745837216566},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T00:00:00Z","timestamp":1453939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI\u201055710","DK\u201046461"],"award-info":[{"award-number":["AI\u201055710","DK\u201046461"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Murine models of <jats:italic>Helicobacter pylori<\/jats:italic> infection are used to study host\u2013pathogen interactions, but lack of severe gastritis in this model has limited its usefulness in studying pathogenesis. We compared the murine gastric epithelial cell line <jats:styled-content style=\"fixed-case\">GSM<\/jats:styled-content>06 to the human gastric epithelial <jats:styled-content style=\"fixed-case\">AGS<\/jats:styled-content> cell line to determine whether similar events occur when cultured with <jats:italic>H. pylori<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The lysates of cells infected with <jats:italic>H. pylori<\/jats:italic> isolates or an isogenic <jats:italic>cagA<\/jats:italic>\u2010deficient mutant were assessed for translocation and phosphorylation of CagA and for activation of stress pathway kinases by immunoblot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Phosphorylated CagA was detected in both cell lines within 60 minutes. Phospho\u2010<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> 1\/2 was present within several minutes and distinctly present in <jats:styled-content style=\"fixed-case\">GSM<\/jats:styled-content>06 cells at 60 minutes. Similar results were obtained for phospho\u2010<jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content>, although the 54 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a phosphoprotein signal was dominant in <jats:styled-content style=\"fixed-case\">AGS<\/jats:styled-content>, whereas the lower molecular weight band was dominant in <jats:styled-content style=\"fixed-case\">GSM<\/jats:styled-content>06 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results demonstrate that early events in <jats:italic>H. pylori<\/jats:italic> pathogenesis occur within mouse epithelial cells similar to human cells and therefore support the use of the mouse model for the study of acute CagA\u2010associated host cell responses. These results also indicate that reduced disease in <jats:italic>H. pylori<\/jats:italic>\u2010infected mice may be due to lack of the Cag <jats:styled-content style=\"fixed-case\">PAI<\/jats:styled-content>, or by differences in the mouse response downstream of the initial activation events.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12300","type":"journal-article","created":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T11:22:05Z","timestamp":1453980125000},"page":"395-404","source":"Crossref","is-referenced-by-count":7,"title":["Early Molecular Events in Murine Gastric Epithelial Cells Mediated by <i>Helicobacter pylori<\/i> CagA"],"prefix":"10.1111","volume":"21","author":[{"given":"Aditi","family":"Banerjee","sequence":"first","affiliation":[{"name":"University of Maryland School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malini","family":"Basu","sequence":"additional","affiliation":[{"name":"Department of Microbiology Rastraguru Surendra Nath College Barrackpore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas G.","family":"Blanchard","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subba R.","family":"Chintalacharuvu","sequence":"additional","affiliation":[{"name":"Covance, Inc. Indianapolis IN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Guang","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik P.","family":"Lillehoj","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J.","family":"Czinn","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198912073212302"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1001\/jama.1994.03520010077036","article-title":"Helicobacter pylori in peptic ulcer disease","volume":"272","author":"Nih CC","year":"1994","journal-title":"J Am Med Assoc"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199110173251603"},{"key":"e_1_2_6_5_1","first-page":"177","volume-title":"Schistosomes, Liver Flukes and Helicobacter pylori","author":"World Health Organization","year":"1994"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/35006081"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/94.22.1680"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01593.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)00899-0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10987"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01271-12"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI64373"},{"key":"e_1_2_6_13_1","first-page":"6735","article-title":"Human gastric carcinogenesis: a multistep and multifactorial process \u2013 first American Cancer Society Award Lecture on Cancer Epidemiology and Prevention","volume":"52","author":"Correa P","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_6_14_1","first-page":"2111","article-title":"Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach","volume":"55","author":"Blaser MJ","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.01.009"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.34781"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14648"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/41483"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.4.593"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2000.00043.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5457.1497"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.25.14559"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.3.1263"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781845935948.0167"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26012-0"},{"issue":"1","key":"e_1_2_6_26_1","first-page":"S160","article-title":"Essential role of urease in vitro and in vivo Helicobacter pylori colonization study using a wild\u2010type and isogenic urease mutant strain","volume":"21","author":"Karita M","year":"1995","journal-title":"J Clin Gastroenterol"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.2022-2031.2003"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.4168-4173.2001"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70155-0"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/IAI.66.4.1349-1355.1998","article-title":"Immune responses of specific\u2010pathogen\u2010free mice to chronic Helicobacter pylori (strain SS1) infection","volume":"66","author":"Ferrero RL","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3529-3538.2002"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.6.3040-3046.1999"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1086\/374780"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2001.02459.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4594-4602.1999"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2534-2541.2003"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.06.047"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060363"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054344"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004450"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1324"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-05-0426"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1083-4389.2004.00226.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-10-2485"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00088.x"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.06.006"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02714.x"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1083-4389.2002.00071.x"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00189.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000684"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06187"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.9.3622-3630.1997"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711183105"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.03.004"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01269.x"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.12.059"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5464-5469.2004"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhel.12300","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hel.12300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T05:09:24Z","timestamp":1695704964000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hel.12300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,28]]},"references-count":56,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1111\/hel.12300"],"URL":"https:\/\/doi.org\/10.1111\/hel.12300","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,28]]}}}