{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T03:57:18Z","timestamp":1771473438069,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,4,8]],"date-time":"2016-04-08T00:00:00Z","timestamp":1460073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aim<\/jats:title><jats:p>We assessed whether adherent gastric mucous to biopsy forceps instead of biopsy samples was suitable for the diagnosis of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> infection. We confirmed the <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> methods to improve the diagnosis of <jats:italic>H. pylori<\/jats:italic> infection and clarithromycin (<jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content>) susceptibility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gastric mucous was obtained by gently scraping gastric mucosa using biopsy forceps in patients undergoing upper gastrointestinal (<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>) endoscopy for <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and rapid urease test (<jats:styled-content style=\"fixed-case\">RUT<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was extracted from gastric mucous present within the gel of <jats:styled-content style=\"fixed-case\">RUT<\/jats:styled-content>. <jats:italic>H. pylori<\/jats:italic> status and <jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content> susceptibility were evaluated using <jats:italic>H. pylori<\/jats:italic>\u2010specific <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> amplification for 23S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> using 4 different primer sets and 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content>. <jats:italic>H. pylori<\/jats:italic> positive was defined as two of the three tests (serum antibody, histology, and <jats:styled-content style=\"fixed-case\">RUT<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) were positive. <jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content> susceptibility was evaluated by point mutations (A 2142G and A 2143G of 23S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Samples taken from 494 subjects were evaluated: 300 <jats:italic>H. pylori\u2010<\/jats:italic>positive patients and 194 negative patients. The results of <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> using <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extracted from gastric mucous present within the <jats:styled-content style=\"fixed-case\">RUT<\/jats:styled-content> gel were consistent with those within water. The accuracy of 23S <jats:styled-content style=\"fixed-case\">rRNA PCR<\/jats:styled-content> for <jats:italic>H. pylori<\/jats:italic> detection using <jats:styled-content style=\"fixed-case\">RUT<\/jats:styled-content> samples was superior to the other tests. The frequency of <jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content> resistance was 38.9%, and eradication rate was 91.3% in the patients with wild\u2010type and 47.0% in the patients with the mutant strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Adherent gastric mucous to biopsy forceps in <jats:styled-content style=\"fixed-case\">RUT<\/jats:styled-content> gel can be used for molecular testing to confirm the diagnosis of <jats:italic>H. pylori<\/jats:italic> infection and for <jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content> susceptibility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12310","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T17:58:42Z","timestamp":1460311122000},"page":"548-553","source":"Crossref","is-referenced-by-count":13,"title":["Molecular Detection of <i>H. pylori<\/i> Using Adherent Gastric Mucous to Biopsy Forceps"],"prefix":"10.1111","volume":"21","author":[{"given":"Hiroshi","family":"Matsumoto","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Shiotani","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Nishibayashi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kawanishi Municipal Hospital Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoari","family":"Kamada","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Kawasaki Medical School Okayama 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