{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:27:58Z","timestamp":1787941678733,"version":"build-2784847793"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T00:00:00Z","timestamp":1465430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Biotechnology, Government of India","award":["(BT\/PR6921\/MED\/29\/699\/2013)"],"award-info":[{"award-number":["(BT\/PR6921\/MED\/29\/699\/2013)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic>, the human gastric pathogen, causes a variety of gastric diseases ranging from mild gastritis to gastric cancer. While the studies on <jats:italic>H.\u00a0pylori<\/jats:italic> are dominated by those based on either East Asian or Western strains, information regarding <jats:italic>H.\u00a0pylori<\/jats:italic> strains prevalent in the Middle East remains scarce. Therefore, we carried out whole\u2010genome sequencing and comparative analysis of three <jats:italic>H.\u00a0pylori<\/jats:italic> strains isolated from three native Arab, Kuwaiti patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p><jats:italic>H. pylori<\/jats:italic> strains were sequenced using Illumina platform. The sequence reads were filtered and draft genomes were assembled and annotated. Various pathogenicity\u2010associated regions and phages present within the genomes were identified. Phylogenetic analysis was carried out to determine the genetic relatedness of Kuwaiti strains to various lineages of <jats:italic>H.\u00a0pylori<\/jats:italic>. The core genome content and virulence\u2010related genes were analyzed to assess the pathogenic potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The three genomes clustered along with HpEurope strains in the phylogenetic tree comprising various <jats:italic>H.\u00a0pylori<\/jats:italic> lineages. A total of 1187 genes spread among various functional classes were identified in the core genome analysis. The three genomes possessed a complete cagPAI and also retained most of the known outer membrane proteins as well as virulence\u2010related genes. The <jats:italic>cagA<\/jats:italic> gene in all three strains consisted of an AB\u2010C type EPIYA motif.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The comparative genomic analysis of Kuwaiti <jats:italic>H.\u00a0pylori<\/jats:italic> strains revealed a European ancestry and a high pathogenic potential.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12323","type":"journal-article","created":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T13:42:40Z","timestamp":1465479760000},"source":"Crossref","is-referenced-by-count":9,"title":["What constitutes an Arabian <i>Helicobacter pylori<\/i>? Lessons from comparative genomics"],"prefix":"10.1111","volume":"22","author":[{"given":"Narender","family":"Kumar","sequence":"first","affiliation":[{"name":"Pathogen Biology Laboratory Department of Biotechnology and Bioinformatics University of Hyderabad  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. John","family":"Albert","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanan","family":"Al Abkal","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqbal","family":"Siddique","sequence":"additional","affiliation":[{"name":"Department of Medicine Faculty of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niyaz","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Pathogen Biology Laboratory Department of Biotechnology and Bioinformatics University of Hyderabad  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01918.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05562"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02967.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080857"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.5230\/jgc.2012.12.2.55"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/315173"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1996.22164006.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01274-0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology2031110"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1433"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4137\/CGast.S13760"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2011.02.003"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002824"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12100"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2014.07.017"},{"key":"e_1_2_8_18_1","first-page":"3","article-title":"Cancer epidemiology and control in the Arab world \u2010 past, present and future","volume":"10","author":"Salim EI","year":"2009","journal-title":"Asian Pac J Cancer Prev"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-10-14"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2006.03144.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-2-6"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030619"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.074492.107"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1226"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08365"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/1.2.199"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.5.955"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm160"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr485"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0524-x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1224503"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki008"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4155-4168.2000"},{"key":"e_1_2_8_37_1","first-page":"189","article-title":"MEGA: Molecular Evolutionary Genetics Analysis software for microcomputers","volume":"10","author":"Kumar S","year":"1994","journal-title":"Comput Appl Biosci"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1086\/508426"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2004.06.014"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2009.06188.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-126"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i46.17618"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01021-13"},{"key":"e_1_2_8_44_1","first-page":"45","article-title":"The bacterial pan\u2010genome: a new paradigm in microbiology","volume":"13","author":"Mira A","year":"2010","journal-title":"Int Microbiol"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002152"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00044-12"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2010.154"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004621"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055120"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhel.12323","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhel.12323","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hel.12323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T19:45:46Z","timestamp":1696275946000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hel.12323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,9]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1111\/hel.12323"],"URL":"https:\/\/doi.org\/10.1111\/hel.12323","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,9]]},"article-number":"e12323"}}