{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:51:20Z","timestamp":1787014280789,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T00:00:00Z","timestamp":1492992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["FCT\u2010PTDC\/BIM\u2010MEC\/1051\/2012"],"award-info":[{"award-number":["FCT\u2010PTDC\/BIM\u2010MEC\/1051\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/95125\/2013"],"award-info":[{"award-number":["SFRH\/BPD\/95125\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2017,8]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Helicobacter pylori<\/jats:italic>\n virulence is associated with different clinical outcomes. The existence of an intact\n dupA<\/jats:italic>\n gene from\n tfs4b<\/jats:italic>\n cluster has been suggested as a predictor for duodenal ulcer development. However, the role of\n tfs<\/jats:italic>\n plasticity zone clusters in the development of ulcers remains unclear. We studied several\n H. pylori<\/jats:italic>\n strains to characterize the gene arrangement of\n tfs3<\/jats:italic>\n and\n tfs4<\/jats:italic>\n clusters and their impact in the inflammatory response by infected gastric cells.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The genome of 14\n H. pylori<\/jats:italic>\n strains isolated from Western patients, pediatric (n=10) and adult (n=4), was fully sequenced using the Illumina platform MiSeq, in addition to eight pediatric strains previously sequenced. These strains were used to infect human gastric cells, and the secreted interleukin\u20108 (\n IL<\/jats:styled-content>\n \u20108) was quantified by\n ELISA<\/jats:styled-content>\n . The expression of\n virB2<\/jats:italic>\n ,\n dupA<\/jats:italic>\n ,\n virB8<\/jats:italic>\n ,\n virB10<\/jats:italic>\n , and\n virB6<\/jats:italic>\n was assessed by quantitative\n PCR<\/jats:styled-content>\n in adherent and nonadherent fractions of\n H. pylori<\/jats:italic>\n during\n in vitro<\/jats:italic>\n co\u2010infection, at different\n pH<\/jats:styled-content>\n values.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n We have found that\n cagA<\/jats:italic>\n \u2010positive\n H. pylori<\/jats:italic>\n strains harboring a complete\n tfs<\/jats:italic>\n plasticity zone cluster significantly induce increased production of\n IL<\/jats:styled-content>\n \u20108 from gastric cells. We have also found that the region spanning from\n virB2<\/jats:italic>\n to\n virB10<\/jats:italic>\n genes constitutes an operon, whose expression is increased in the adherent fraction of bacteria during infection, as well as in both adherent and nonadherent fractions at acidic conditions.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n A complete\n tfs<\/jats:italic>\n plasticity zone cluster is a virulence factor that may be important for the colonization of\n H. pylori<\/jats:italic>\n and to the development of severe outcomes of the infection with\n cagA<\/jats:italic>\n \u2010positive strains.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/hel.12390","type":"journal-article","created":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T07:39:50Z","timestamp":1493019590000},"source":"Crossref","is-referenced-by-count":24,"title":["The expression of\n Helicobacter pylori tfs<\/i>\n plasticity zone cluster is regulated by\n pH<\/scp>\n and adherence, and its composition is associated with differential gastric\n IL<\/scp>\n \u20108 secretion"],"prefix":"10.1111","volume":"22","author":[{"given":"Bruno","family":"Silva","sequence":"first","affiliation":[{"name":"National Reference Laboratory for Gastrointestinal Infections Department of Infectious Diseases National Institute of Health Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Nunes","sequence":"additional","affiliation":[{"name":"Bioinformatics Unit Department of Infectious Diseases National Institute of Health Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4635-0105","authenticated-orcid":false,"given":"Filipa F.","family":"Vale","sequence":"additional","affiliation":[{"name":"Host\u2010Pathogen Interactions Unit Research Institute for Medicines (iMed\u2010ULisboa) Instituto de Medicina Molecular Faculdade de Farm\u00e1cia da Universidade de Lisboa Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Rocha","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Gastrointestinal Infections Department of Infectious Diseases National Institute of Health Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Paulo","family":"Gomes","sequence":"additional","affiliation":[{"name":"Bioinformatics Unit Department of Infectious Diseases National Institute of Health Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Dias","sequence":"additional","affiliation":[{"name":"Biosystems and Integrative Sciences Institute (BioISI) Faculdade de Ci\u00eancias Universidade de Lisboa Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6360-2576","authenticated-orcid":false,"given":"M\u00f3nica","family":"Oleastro","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Gastrointestinal Infections Department of Infectious Diseases National Institute of Health Lisbon 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